Method for screening and identifying hybrid generations with deletion of soybean lipoxygenase and 7S and 11S globulin subunits.

Abstract

The invention discloses a method for screening and identifying hybrid generations with deletion of soybean lipoxygenase and 7S and 11S globulin subunits. According to the invention, by changing the operation process, the reagent components and the ratio, accurate identification of four characters possibly occurring in filial generation, such as simultaneous existence, simultaneous deletion, Lox-3 deletion, Lox-1 deletion and Lox-2 deletion of three isoenzymes Lox-1, Lox-2 and Lox-3 of lipoxygenase, is realized through one-time trace sampling; the defect that various characters of lipoxygenase deletion cannot be identified through color difference due to the fact that beta-carotene is extremely easy to oxidize is overcome, and the defect that whether a certain strip is deleted or not cannot be judged due to the fact that the distance between three strips Lox-1, 2 and 3 is too close in the existing process of detecting whether the three target characters Lox-1, 2 and 3 are deleted or not is overcome; by utilizing residues after lipoxygenase identification, synchronous identification of deletion of each subunit of 7S and 11S globulins is realized without increasing sampling times.

Description

technical field [0001] The invention relates to a screening and identification method for soybean seeds, in particular to a screening and identification method for soybean lipoxygenase and 7S, 11S globulin subunit deletion hybrid progeny, and belongs to the field of screening and identification of soybean seeds. Background technique [0002] The trait of lipoxygenase deletion is controlled by double recessive genes, the deletion of three subunits of 11S globulin is a pure recessive trait, and the deletion of 7S is controlled by a single dominant gene. Therefore, in the process of soybean breeding, whether lipoxygenase, 7S, 11S globulin subunits are missing or not, traits will segregate in the F2 generation, that is, theoretically in the F2 generation, seeds containing the missing traits can be screened out, so that Obtaining the target seeds in the early generation has the advantage of reducing the detection workload and detection cost, preventing the geometric progression o...

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Problems solved by technology

In addition, this method also has the following defects: 1. It is difficult to distinguish protein subunits with similar molecular weights, and there is such a problem in the identification of lipoxygenase deletion. Since lipoxygenase is composed of three isoenzymes, its molecular weight is between 94 Between -97Kd, reflected on the SDS-PAGE gel, their bands are very close and almost fall together, it is easier to identify three isoenzyme deletions at the same time, but it is more difficult to identify the deletion of one of the enzymes, there are errors The disadvantage of high judgment rate; 2. The detection cycle is long, and it takes at least 12 hours for one cycle

3. The amount of sampling is large, the seeds are seriously damaged, and it is easy to cause mildew during the soil germination process, which cannot ensure the safe emergence of seeds containing t

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