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Immune metabolic myocardial infarction patch and preparation method and application thereof

A patch and myocardial infarction technology, applied in the field of immunometabolic myocardial infarction patch and its preparation, can solve problems such as difficulty in determining the drug site

Active Publication Date: 2021-12-31
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of injection makes it difficult to determine the site of administration, and there are obvious side effects

Method used

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  • Immune metabolic myocardial infarction patch and preparation method and application thereof
  • Immune metabolic myocardial infarction patch and preparation method and application thereof
  • Immune metabolic myocardial infarction patch and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Reducing the proportion of inflammatory macrophages in the myocardial infarction area

[0031] On the 3rd day after the operation, the heart was taken to prepare frozen sections, and the tissue sections of macrophages were immunofluorescently stained, iNOS was marked in green, F4 / 80 was marked in red, and nuclei were marked in blue by DAPI. See the experimental results figure 1 , *P < 0.05, **P < 0.01, ***P < 0.001.

Embodiment 2

[0032] Example 2 Increase the survival of mesenchymal stem cells

[0033] Mesenchymal stem cells (Fluc-MSCs) transfected with luciferase gene lentivirus were prepared by conventional methods, and the survival rate of transplanted mesenchymal stem cells was observed by using luciferase to react with substrate to produce bioluminescent properties. Specifically: on the 1st, 3rd, and 7th day after the operation, live imaging detection was performed on the mice to observe the bioluminescent signal (BLI) in the heart of the mice. , continuously detect the BLI signal for 10 minutes, and measure it every 1 minute until the signal reaches the peak. See the experimental results figure 2 , *P<0.05, **P<0.01, ***P<0.001.

Embodiment 3

[0034] Example 3 Effectively improve cardiac function after myocardial infarction

[0035] Cardiac function after myocardial infarction by echocardiography. The mice were anesthetized (the same method as before), placed in the left lateral position after hair removal, placed the probe of the cardiac ultrasound diagnostic instrument on the anterior wall of the heart, and took a two-dimensional short-axis view of the left ventricle at the level of the papillary muscle, and recorded M-mode scans at the same time, for 3 consecutive Cardiac cycle measurements of left ventricular ejection fraction (EF) and fractional shortening (FS). See the experimental results image 3 ,* P < 0.05.

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Abstract

The invention discloses an immune metabolic myocardial infarction patch and a preparation method and application thereof. The immune metabolic myocardial infarction patch can be used as a biological material for treating myocardial infarction by synergistic stem cells. Animal experiments prove that after a 2-deoxy-D-glucose patch is applied, the survival rate of injected mesenchymal stem cells is increased, the treatment effect of the mesenchymal stem cells is enhanced, and the side effect of injecting 2-deoxy-D-glucose is reduced.

Description

technical field [0001] The invention belongs to biological material technology, and relates to an immunometabolic myocardial infarction patch and its preparation method and application, in particular to the application of 2-deoxy-D-glucose patch in the preparation of biological materials for treating myocardial infarction with synergistic stem cells. Background technique [0002] Myocardial infarction (MI) is a cardiovascular disease that seriously endangers human health. With the continuous improvement of people's living standards in our country, the incidence of ischemic myocardial infarction is also increasing. Ischemic myocardial infarction leads to necrosis and scarring of cardiomyocytes, which in turn affects cardiac function. Most of the current drug or device treatments can only relieve the symptoms, but they cannot reverse the damage to the heart tissue. Although heart transplantation can completely improve the state of the heart, it is difficult to be widely used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K31/7004A61K47/36A61K47/42A61P9/10
CPCA61K9/0024A61K47/36A61K47/42A61K31/7004A61P9/10A61K9/7007
Inventor 沈振亚陈维倩肖威章陈明
Owner SUZHOU UNIV