Method for preparing bispecific antibody by using circular-track shaking type bioreactor

A bispecific antibody and bioreactor technology, applied in biochemical equipment and methods, chemical instruments and methods, biochemical instruments, etc., can solve the problems of difficult amplification, huge shear force, death, etc., and prolong the time of cell culture , high protein expression, and high cell viability

Pending Publication Date: 2021-12-31
GUANGZHOU LINTONPHARM CO LTD +3
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the use of stirred bioreactors to cultivate animal cells to produce monoclonals has some defects. First, during the scale-up culture process, the shear force caused by the stirring paddles will cause damage to the cells, or even death
Secondly, the stirred bioreactor generally adopts

Method used

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  • Method for preparing bispecific antibody by using circular-track shaking type bioreactor
  • Method for preparing bispecific antibody by using circular-track shaking type bioreactor
  • Method for preparing bispecific antibody by using circular-track shaking type bioreactor

Examples

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Example Embodiment

[0068] Example 1

[0069] A method of cultured cells using a loop-shaking biological reactor, and the cell line was seeded in a medium in a medium in a medium in a medium. Comparative Example 1, Comparative Example 2 and Example 1 Culture Cell conditions are shown in Table 1.

[0070] Table 1 Comparative Example 1-2 and Example 1 Culture Conditions

[0071]

[0072]

[0073] N / A is not applicable.

[0074] PH Control Strategy: Comparative Examples 1, 2 and Example 1 Monitor the medium pH in real time, if pH is lower than the above pH range, add 1M NaHCO 3 Elevated pH, if the pH is lower than the above pH range, enter the CO 2 Reduce the pH.

[0075] DO Control Strategy: The contrast 1, 2 is introduced into pure oxygen lifting oxygen, and in Example 1 Example 1 was transferred into air and oxygen heavily dissolved oxygen.

[0076] During the culture, cell density and activity were detected using a cell count (Bakerman company), using Octetred96 to detect antibody titers.

[0...

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Abstract

The invention discloses a method for preparing a bispecific antibody by using a circular-track shaking type bioreactor. A rotating speed and pH of cell culture conditions and other culture conditions are adjusted. Results show that the cell culture method disclosed by the invention has the advantages that relatively high cell viability can be kept for a long time, and the culture time is prolonged; and relatively high cell density and relatively-long-time high cell viability can be obtained by prolonging the culture time in the circular-track shaking type reactor.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for preparing bispecific antibodies using a ring-orbit shaking bioreactor. Background technique [0002] Antibodies have high stability in the blood and few side effects, so they are attracting more and more attention as drugs. Bispecific antibodies refer to antibodies that can recognize two antigens (antigen A and antigen B) at the same time. At present, bispecific antibodies are mainly expressed and produced by in vitro culture of animal cells. Stirred bioreactor is a commonly used reactor in the field of cell culture. The technology is relatively mature, but there are still many problems, which will bring obstacles to the process of cell scale-up culture. First of all, during the scale-up of cell culture, the shear force caused by the stirring paddles will cause damage to the cells, even death. Secondly, the stirred bioreactor generally adopts the deep ventilation ...

Claims

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Application Information

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IPC IPC(8): C07K16/46C12P21/00
CPCC07K16/468C07K16/2809C07K16/2887C07K16/32C07K16/2863C07K2317/14C07K2317/31C12M27/16C07K2317/10C12P21/02
Inventor 陆金康景涛吴小波胡飞
Owner GUANGZHOU LINTONPHARM CO LTD
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