Method for preparing bispecific antibody by using circular-track shaking type bioreactor
A bispecific antibody and bioreactor technology, applied in biochemical equipment and methods, chemical instruments and methods, biochemical instruments, etc., can solve the problems of difficult amplification, huge shear force, death, etc., and prolong the time of cell culture , high protein expression, and high cell viability
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[0068] Example 1
[0069] A method of cultured cells using a loop-shaking biological reactor, and the cell line was seeded in a medium in a medium in a medium in a medium. Comparative Example 1, Comparative Example 2 and Example 1 Culture Cell conditions are shown in Table 1.
[0070] Table 1 Comparative Example 1-2 and Example 1 Culture Conditions
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[0072]
[0073] N / A is not applicable.
[0074] PH Control Strategy: Comparative Examples 1, 2 and Example 1 Monitor the medium pH in real time, if pH is lower than the above pH range, add 1M NaHCO 3 Elevated pH, if the pH is lower than the above pH range, enter the CO 2 Reduce the pH.
[0075] DO Control Strategy: The contrast 1, 2 is introduced into pure oxygen lifting oxygen, and in Example 1 Example 1 was transferred into air and oxygen heavily dissolved oxygen.
[0076] During the culture, cell density and activity were detected using a cell count (Bakerman company), using Octetred96 to detect antibody titers.
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