Method for preparing bispecific antibody by using circular-track shaking type bioreactor

Abstract

The invention discloses a method for preparing a bispecific antibody by using a circular-track shaking type bioreactor. A rotating speed and pH of cell culture conditions and other culture conditions are adjusted. Results show that the cell culture method disclosed by the invention has the advantages that relatively high cell viability can be kept for a long time, and the culture time is prolonged; and relatively high cell density and relatively-long-time high cell viability can be obtained by prolonging the culture time in the circular-track shaking type reactor.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for preparing bispecific antibodies using a ring-orbit shaking bioreactor. Background technique [0002] Antibodies have high stability in the blood and few side effects, so they are attracting more and more attention as drugs. Bispecific antibodies refer to antibodies that can recognize two antigens (antigen A and antigen B) at the same time. At present, bispecific antibodies are mainly expressed and produced by in vitro culture of animal cells. Stirred bioreactor is a commonly used reactor in the field of cell culture. The technology is relatively mature, but there are still many problems, which will bring obstacles to the process of cell scale-up culture. First of all, during the scale-up of cell culture, the shear force caused by the stirring paddles will cause damage to the cells, even death. Secondly, the stirred bioreactor generally adopts the deep ventilation ...

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the use of stirred bioreactors to cultivate animal cells to produce monoclonals has some defects. First, during the scale-up culture process, the shear force caused by the stirring paddles will cause damage to the cells, or even death

Secondly, the stirred bioreactor generally adopts the deep ventilation method, which is easy to generate foam, and at the same time, the burst of the bubble will cause a greater shear force

In addition, during the expansion of the stirred bioreactor, due to the many parameters involved and the complex fluid mechanics, it is difficult to scale up or even fail.

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