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Colorectal cancer diagnostic marker and application thereof

A technology for early diagnosis of colorectal cancer, applied in the field of molecular biology, to achieve the effects of improved specificity, high sensitivity and high specificity

Active Publication Date: 2021-12-31
SHANGHAI TONGJI HOSPITAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report about a diagnostic marker for colorectal cancer of the present invention and its application

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  • Colorectal cancer diagnostic marker and application thereof
  • Colorectal cancer diagnostic marker and application thereof
  • Colorectal cancer diagnostic marker and application thereof

Examples

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Embodiment 1

[0035] Example 1 Screening of exosomal proteins CPS1 and TNC

[0036] 1 In the present invention, firstly, the plasma exosome samples of colorectal cancer patients obtained from Shanghai Tongji Hospital are processed and analyzed for label-free protein spectrum detection. According to the protein spectrum processing and analysis process control method, import protein spectrum data, and obtain theoretically effective proteins after signal value screening and standardization, and perform differential expression analysis on this basis, see figure 1 .

[0037] 2 The protein screening method specifically includes the following steps:

[0038] 2.1 Collect plasma exosome samples from patients with colorectal cancer and extract total protein.

[0039] 2.2 Perform quality detection and labeling on the obtained protein.

[0040] 2.3 The label-free protein spectrum detection technology of Jingjie Company was used to detect and analyze under standard conditions.

[0041] 2.4 Perform q...

Embodiment 2

[0047] Example 2 Method for diagnosing CRC based on exosomal protein CPS1 and TNC

[0048] 1 Serum exosomes were extracted according to conventional methods.

[0049] 2 Extract exosomal proteins CPS1 and TNC according to conventional methods.

[0050] 3According to the diagnostic threshold of 0.655, if the relative expression of CPS1 combined with TNC in serum exosomes relative to the internal reference protein Actin is >0.655, it means that CRC is present; otherwise, if the relative expression of CPS1 combined with TNC in serum exosomes relative to the internal reference protein Actin If the expression level is ≤0.655, then there is no CRC.

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Abstract

The invention relates to the technical field of molecular biology, in particular to a colorectal cancer diagnostic marker and application thereof. Standard protein expression profile data is obtained by performing label-free protein spectrum detection data processing and analysis on the plasma exosome of a colorectal cancer patient, and then differentially expressed protein-CPS1 and TNC are screened out. Then, the expression difference of CPS1 and TNC in colorectal cancer and para-carcinoma tissue samples and patient serum samples is detected through experiments, the result shows that the expression levels of CPS1 and TNC in colorectal cancer tissue are obviously higher than the expression level in para-carcinoma tissue, and the experiment result also shows that the expression levels of CPS1 and TNC are in positive correlation with advanced and lymph node metastasis and distant metastasis; the marker can be used as an index for early screening and prognosis evaluation of colorectal cancer. The specificity and the sensitivity of combined use of the two are remarkably improved compared with those of independent use of the two, and the advantages of noninvasive property, convenience and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a diagnostic marker for colorectal cancer and its application. Background technique [0002] Colorectal cancer (CRC) is one of the most common digestive tract malignancies. According to the World Health Organization's International Agency for Research on Cancer, its morbidity and mortality rate account for the top three of all malignant tumors. With the improvement of my country's economy and life in the past 30 years, the incidence and death of colorectal cancer in my country are on the rise, ranking third in the incidence of malignant tumors and fifth in death in my country, seriously threatening people's health and life . Colorectal cancer has obvious regional distribution differences in the world. The high-incidence areas are mainly Australia, New Zealand, Europe, and North America, while the incidence rates are low in Africa, Central and South Asia, and other area...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57419G01N33/57488G01N33/68
Inventor 李冬商安全孙祖俊王旋谷晨峥吴军录权文强
Owner SHANGHAI TONGJI HOSPITAL