Low-temperature rotary blending box

Abstract

The invention belongs to the technical field of biology, and particularly discloses a low-temperature rotary blending box which comprises a heat preservation box body and a ball body. The ball body comprises an upper ball body and a lower ball body which are detachable; an opening is formed in the upper portion of the heat preservation box body, a fixedly-installed ice box is arranged in the heat preservation box body, and ice is arranged in the ice box. After a standard EP tube is inserted into the ball body, the heat preservation box body is placed on a shaking table, the ball body continuously rolls along with shaking of the shaking table, and a target protein solution of the standard EP tube in the ball body is continuously shaken, so that the target protein solution is uniformly mixed. According to the scheme, a target protein solution in a standard EP tube can be uniformly shaken, and the experiment cost is relatively low.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a low-temperature rotary mixing box. Background technique [0002] Co-immunoprecipitation (Co-Immunoprecipitation) is a classic method for studying protein interaction based on the specific interaction between antibody and antigen, and is an effective method to determine the physiological interaction of two proteins in intact cells. It is a method developed by utilizing the specific binding of antigen and antibody and the phenomenon that protein A or G of bacteria specifically binds to the Fc fragment of immunoglobulin. The basic principle is to add an antibody against the protein of interest to the cell lysate, and then add Protein A or G that binds to the Agarose beads that specifically binds to the antibody after incubation. If there is a target protein that binds to the protein of interest in the cell, then Such a complex can be formed: "Protein of interest-antibody protein anti...

AI Technical Summary

Problems solved by technology

[0004] Among them, in step (2), in order to ensure that the protein is fully bound, it can be slowly shaken for a longer time or even overnight according to the needs of the schedule. In the experiment, a shaker is often used to shake the target protein solution. However, the existing Most of the shaking tables in the market only shake the target protein solution in one direction (left and right). The target protein solution is only subjected to force in one direction and the shaking is uneven. The shaker that can shake the target protein solution at 360° has a high cost and the purpose The protein solution needs to be shaken at 4°C. Most shakers cannot meet the low temperature environment of the target protein solution, and the purchase cost of the low temperature shaker is relatively high. For example, the vertical full temperature shaking shaker model JYC-2102C, Its purchase price is about 38,500. In order to meet the low-temperature shaking environment of the target protein solution, the shaker can also be placed in the special refrigerator for the experiment, but the price of the special refrigerator is expensive. For example, the low-temperature refrigerator with the product number H0059, Its purchase cost is about 9092 yuan, and the experiment cost is relatively high

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