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Application of urine protein marker in preparation of kit for detecting clinical stages of diabetic nephropathy

A technique for diabetic nephropathy and clinical staging, which is applied in the field of diabetic nephropathy and can solve problems such as deviation

Pending Publication Date: 2022-01-11
北京松果天目健康管理有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gold standard for the clinical diagnosis of DKD is renal biopsy, however, it is highly invasive and dangerous, and the evaluation process may also be biased by subjectivity

Method used

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  • Application of urine protein marker in preparation of kit for detecting clinical stages of diabetic nephropathy
  • Application of urine protein marker in preparation of kit for detecting clinical stages of diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Part I: Urine Protein Sample Preparation

[0062] Example 1 of an invention patent application whose publication number is CN 108333263 A and whose publication date is July 27, 2018. The preparation method is prepared.

[0063] Part II: Mass Spectrometry Detection

[0064] Digested samples were separated from tryptic peptides on a homemade capillary column packed with C18 particles and analyzed by a Thermo Fisher Orbitrap mass spectrometer coupled to an online Easy-nLC 1000 Nano-HPLC system (Thermo Fisher Scientific). LC-MS / MS data were processed in the FIRMIANA analysis platform [Nat Biotechnol.2017 May 9;35(5):409-412.], and protein searches were performed on the Mascot search engine.

[0065] Protein abundance was measured as iBAQ (Intensity-Based Relative Quantification)-label-free quantification algorithm. For batch-to-batch comparisons, iBAQ was converted to iFOT (intensity-based total fraction), representing the normalized intensity of proteins identified in t...

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Abstract

The invention provides application of a urine protein marker in preparation of a kit for detecting clinical stages of diabetic nephropathy. The urine protein marker is one or more of the following proteins in urine: ANXA7, APOD and C9, and the clinical stage is from stage 3 to stage 4. A large amount of proteins in urine of a clinical third-stage patient and a clinical fourth-stage patient of the diabetic nephropathy are detected by utilizing a mass-spectrometric technique, and the three proteins ANXA7, APOD and C9 are found to have significant differences between the clinical stage 3 patient and the clinical stage 4 patient of the diabetic nephropathy. Therefore, the three proteins in the urine have important clinical application value as markers for detecting clinical stages 3-4 of diabetic nephropathy.

Description

technical field [0001] The invention relates to the field of diabetic nephropathy, in particular to the application of a urinary protein marker in the preparation of a kit for detecting the clinical stages of diabetic nephropathy. Background technique [0002] In 2019, more than 463 million people worldwide suffered from diabetes, accounting for 9.3% of global adults (20-79 years old). Among these populations, 20%-40% will develop diabetic kidney disease (DKD), which is the leading cause of morbidity and mortality in type 2 diabetes (T2D) patients. DKD patients are at high risk of developing end-stage renal disease (ESRD) and cardiovascular disease. According to the internationally recognized Mogensen staging, DKD is divided into five stages, and stages 1-3 are still in the reversible stage (see the table below). Therefore, early detection, prevention and treatment are very important. The gold standard for the clinical diagnosis of DKD is renal biopsy, but it is highly in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/577G01N33/58G01N27/62G16H50/20
CPCG01N33/6893G01N33/54306G01N33/54313G01N33/577G01N33/582G01N33/581G01N33/587G01N33/6848G16H50/20G01N2800/042G01N2800/347G01N2800/56G01N2800/60
Inventor 汪宜范冠杰秦钧宫童庆林玉平
Owner 北京松果天目健康管理有限公司