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Application of EGCG quinone as inhibitor for resisting release of AGEs in gastrointestinal tract

A gastrointestinal tract and inhibitor technology, applied in the application field of EGCG quinone, to achieve the effect of large market application value

Pending Publication Date: 2022-01-14
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not evaluated as an inhibitor against the release of AGEs in the gastrointestinal tract

Method used

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  • Application of EGCG quinone as inhibitor for resisting release of AGEs in gastrointestinal tract
  • Application of EGCG quinone as inhibitor for resisting release of AGEs in gastrointestinal tract
  • Application of EGCG quinone as inhibitor for resisting release of AGEs in gastrointestinal tract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Experiments on the activity of EGCG quinone against AGEs released in the gastrointestinal tract:

[0029] 1. Test content:

[0030] Analysis of the anti-AGEs release activity of EGCG quinone in the gastrointestinal tract: Combined with the determination of AGEs in vitro and the hydrolysis degree of glycated protein and multi-spectral detection method, the anti-AGEs release activity of EGCG quinone in the gastrointestinal tract was evaluated and its mechanism was studied Research.

[0031] 2. Test method:

[0032] 2.1 Preparation of glycosylated protein

[0033] In the glycation model system, β-lactose (β-Lactose) and casein (Casein) are used to prepare glycated protein. Saccharification was performed in phosphate buffer (100 mM, pH 7) containing 5 mg / mL Casein and 8 mg / mL β-Lactose. The mixed solution was heated in a water bath at 98°C ± 2°C for 2 hours. The heated samples were dialyzed at 4°C for 3 days using a 3 kDa MW dialysis belt to remove excess small molecul...

Embodiment 2

[0051] Experiments on the influence of EGCG quinone on the digestibility of glycated protein:

[0052] 1. Test content

[0053] After the glycosylated protein undergoes gastrointestinal simulated digestion in vitro, the degree of hydrolysis of the glycosylated protein at different stages of digestion is further detected to study the inhibitory effect of EGCG quinone on the hydrolysis of the glycosylated protein.

[0054] 2. Test method

[0055] Digested samples (75 μL) were mixed with 75 μL of 24% TCA and precipitated on ice for 30 minutes. The solution was then centrifuged at 13,000 rpm for 20 minutes at 4°C. After centrifugation, 30 μL of standard (L-leucine) or sample supernatant was removed and mixed with 900 μL of sodium tetraborate (0.1 M, pH 8.0). Next, 300 μL of fluorescamine solution (0.2 mg / mL) was added to the above solution, and fluorescence was measured using excitation and emission wavelengths of 390 and 480 nm, respectively. DH is calculated as follows (Equa...

Embodiment 3

[0063] Interaction test of EGCG quinone on glycated protein:

[0064] 1. Test content

[0065] The interaction of EGCG quinones with glycated proteins in simulated gastrointestinal tract environment was detected by fluorescence spectroscopy, circular II and infrared spectroscopy.

[0066] 2. Test method

[0067] 2.1 Fluorescence Spectrum

[0068] Using a Hitachi F-4700 fluorescence spectrophotometer (Tokyo, Japan), the influence of EGCG quinone on the intrinsic fluorescence spectrum of glycated protein was determined. For the intrinsic spectrum, at the excitation wavelength of 280nm, the emission spectra of protein solutions at 295-450nm at 10umol / L under different digestion environments were recorded. The slit width for excitation and emission is 5.0 nm. All samples were scanned three times and the data averaged.

[0069] 2.2 Circular Dichroism

[0070] Glycated protein at 0.1 mg / ml was mixed with EGCG quinone. All samples were incubated at 37°C for 30 min. Then the s...

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Abstract

The invention relates to the technical field of application of EGCG quinone, and particularly relates to application of EGCG quinone as an inhibitor for resisting release of AGEs in the gastrointestinal tract. The EGCG quinone has the activity of resisting release of the AGEs in the gastrointestinal tract. The EGCG quinone is specifically capable of effectively inhibiting release of the AGEs in the gastrointestinal tract, and comprises a representative CML of fluorescent AGEs and non-fluorescent AGEs. The related mechanism comprises interaction between the EGCG quinone and glycosylated protein and digestive enzyme, and is capable of inhibiting the activity of the digestive enzyme and changing the conformation of the glycosylated protein. In addition, the EGCG quinone also has a considerable scavenging effect on the released AGEs. Therefore, the EGCG quinone has the potential to serve as the inhibitor for resisting release of the AGEs in the gastrointestinal tract, can serve as a functional food additive, is used for developing functional food for resisting release of the AGEs in the gastrointestinal tract, and has great market application value.

Description

technical field [0001] The invention relates to the technical field of EGCG quinone application, in particular to the application of EGCG quinone as an inhibitor against the release of AGEs in the gastrointestinal tract. Background technique [0002] Glycation of dietary proteins during food processing leads to the formation of potentially harmful substances such as advanced glycation end products (AGEs). According to different sources, AGEs mainly include two types: endogenous AGEs and food-derived AGEs. Among them, two-thirds of the orally absorbed food-derived AGEs (10% of the intake) are retained in human tissues in the form of endogenous substances, and the rest are excreted through the kidneys, and about 50-60% of the free AGEs in the diet Excretion after ingestion, whereas protein-bound AGEs have a limited excretion rate. Glycated protein is the most common source of dietary AGEs. Before reaching the small intestine, protein-bound AGEs can only be released after enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
CPCA23L33/105A23V2002/00A23V2250/2132A23V2200/30
Inventor 吴茜欧阳宇冯年捷周梦舟徐宁汪超
Owner HUBEI UNIV OF TECH
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