Preeclampsia diagnostic kit and application of fluorescent dye

A diagnostic kit and preeclampsia technology, applied in fluorescence/phosphorescence, preparation of test samples, material analysis through optical means, etc., can solve the basic research problems of ignoring the pathological changes of the placenta

Pending Publication Date: 2022-01-14
SHENZHEN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, previous studies have focused too much on specific proteins or factors associated with preeclampsia, while neglecting fundamental studies of placental pathology

Method used

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  • Preeclampsia diagnostic kit and application of fluorescent dye
  • Preeclampsia diagnostic kit and application of fluorescent dye
  • Preeclampsia diagnostic kit and application of fluorescent dye

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The peripheral blood of three normal pregnant women, the peripheral blood of mild preeclampsia pregnant women and the peripheral blood of severe preeclampsia pregnant women were provided respectively, which were recorded as N1 (peripheral blood of normal pregnant women 1), N2 (peripheral blood of normal pregnant women 2), and N3 (Peripheral blood of normal pregnant women 3), M (peripheral blood of pregnant women with mild preeclampsia), S (peripheral blood of pregnant women with severe preeclampsia).

[0042] Take part of N1, M, and S peripheral blood samples respectively, add anticoagulant (trisodium citrate) respectively, drop them on glass slides to dry, then drop them into undiluted Nile blue dye for staining, and use the gradually diffused dye The whole blood was stained, dried, and then smeared with neutral gel to obtain whole blood test samples, which were respectively recorded as N1-whole blood, M-whole blood, and S-whole blood.

[0043] N1-whole blood, M-whole ...

Embodiment 2

[0048] Parts of N1, N2, N3, M, and S peripheral blood samples were taken and centrifuged at 1500rpm for 10 minutes, and then serum was collected and recorded as N1-serum, N2-serum, N3-serum, M-serum, and S-serum.

[0049] The following two methods were used respectively to process the above five groups of serum samples to obtain serum test samples.

[0050] The first method: mix Nile blue and serum at a ratio of 1:9 by volume, and then spread them evenly and thinly on glass slides, and seal them with neutral resin after drying.

[0051] The second method: mix Nile blue and serum at a ratio of 1:9 by volume, and then apply them evenly and thickly, and seal them with neutral resin after drying.

[0052] The obtained serum test samples were recorded as N1.1-serum, N1.2-serum, N2.1-serum, N2.2-serum, N3.1-serum, N3.2-serum, M1-serum, M2-serum Serum, S1-serum, S2-serum.

[0053] The FLIM system was used to carry out diagnostic research on the above serum test samples respectively...

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Abstract

The invention discloses a preeclampsia diagnostic kit and application of nile blue. The preeclampsia diagnostic kit comprises a fluorescent dye for dyeing a blood sample of a pregnant woman. According to the preeclampsia diagnostic kit, a pregnant woman blood sample is dyed through a fluorescent dye, a normal pregnant woman blood sample and a preeclampsia pregnant woman patient blood sample are treated through the fluorescent dye in combination with the test of the embodiment part, by combining the FLIM technology, a blood sample of a normal pregnant woman and a blood sample of a preeclampsia pregnant woman patient can be obviously distinguished so that the preeclampsia can be diagnosed.

Description

technical field [0001] The invention relates to the field of diagnostic kits, in particular to a diagnostic kit for preeclampsia and the application of fluorescent dyes. Background technique [0002] Preeclampsia (PE), characterized by hypertension and edema, is a serious pregnancy complication that can lead to maternal and perinatal death. Studies have shown that placental dysplasia and the emergence of certain pathogenic factors may lead to preeclampsia. Previous studies have paid attention to the pathogenic factors that induce preeclampsia. It is believed that on the basis of these studies, an early diagnosis method for preeclampsia can be developed by detecting specific biomarkers in the blood of pregnant women. The placental model established by G.J.Burton et al. showed that dysplasia of spiral arteries can lead to excessive blood flow into the maternal-fetal interface, resulting in placental villus damage and the production of inflammatory factors. However, studies o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N21/64
CPCG01N1/30G01N21/6428G01N2021/6439
Inventor 张慧严伟朱殷铷吴本清王金章屈军乐
Owner SHENZHEN UNIV
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