Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography

An immunofluorescence and quantitative technique, which is applied in the field of biology and immunoassay, can solve the problems of inability to operate on-site, high cost of detection, complicated pretreatment, etc., and achieve the effect of convenient detection, convenient observation, and good repeatability of results

Pending Publication Date: 2022-01-14
QINGDAO HIGHTOP BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned method is sensitive, accurate, highly specific, and well-separated, and can simultaneously determine multiple drugs, but requires expensive instruments, complicated sample pretreatment, tedious and time-consuming, high cost of detection, cannot be operated on site, and requires professional expertise. Personnel operation, long detection time

Method used

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  • Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography
  • Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography
  • Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Such as Figure 1-3 As shown, a test card for the quantitative detection of folic acid by immunofluorescence chromatography, including an upper cover 1 and a lower cover 2, the upper cover 1 and the lower cover 2 are snap-connected to each other, and an accommodation chamber is formed between the upper cover 1 and the lower cover 2 , a test strip is set in the chamber, and the test strip includes a PVC bottom plate 3, a sample pad 4, a blood filter pad 5, a first binding pad 6, a second binding pad 7, a nitrocellulose membrane 8 and an absorbent paper 11, The sample pad 4, the blood filter pad 5, the first binding pad 6, the second binding pad 7, the nitrocellulose membrane 8 and the water-absorbing paper 11 are sequentially overlapped with each other and then pasted on the PVC base plate 3, and overlapped with each other. The size of the sample pad 4 is 1mm, one end of the sample pad 4 is pasted on the PVC base plate 3, the other end of the sample pad 4 is lapped on on...

Embodiment 2

[0067] A kit for the quantitative detection of folic acid by immunofluorescence chromatography, the kit includes a sample dissociation solution and a detection card as described in Example 1, and the sample dissociation solution includes a stabilizer, a denaturant and a neutralizer , stabilizers include 25mM MES, 1% TCEP, 0.5% mercaptoethanol, 0.2% PC300 and 2.5% sodium ascorbate, denaturants include 0.1mM sodium carbonate-sodium bicarbonate buffer, 5% sodium hydroxide, 0.1% methanol and 0.2 %PC300, the neutralizer contains 50mM Tris buffer, 0.9% NaCl and 0.5% casein, and the above percentages are all mass percentages.

[0068] The detection method of the kit is:

[0069] The sample to be tested is serum, whole blood or plasma, and the sample is dissociated with the sample dissociation solution. The sample dissociation process is: mix 25 μL of the stabilizer and 15 μL of the denaturant, add 40 μL of the sample to be tested, let stand at 37°C for 5 minutes, add Mix 50 μL of th...

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Abstract

The invention relates to a detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography, and belongs to the technical field of biology and immunoassay. The detection card comprises an upper cover and a lower cover, a containing cavity is formed between the upper cover and the lower cover, and a test strip is arranged in the containing cavity. The test strip comprises a PVC base plate, a sample pad, a blood filtering pad, a first conjugate pad, a second conjugate pad, a nitrocellulose membrane and absorbent paper. The sample pad, the blood filtering pad, the first conjugate pad, the second conjugate pad, the nitrocellulose membrane and the absorbent paper are sequentially and mutually overlapped in a staggered mode and then pasted on the PVC base plate. The nitrocellulose membrane is sequentially coated with a detection T line and a quality control C line. The first conjugate pad and the second conjugate pad are both coated with solid-phase liquid, folic acid binding protein which is marked by fluorescent substances and has the concentration of 1 mg / mL and folic acid-BSA marked by biotin substances. The detection card and the kit are used for rapidly detecting folic acid in batches, and are high in detection sensitivity, good in specificity, rapid and accurate in detection, convenient and fast to use, easy to operate, convenient to carry, convenient to store and transport.

Description

technical field [0001] The invention relates to the technical fields of biology and immunoassay, in particular to a detection card and a kit for quantitative detection of folic acid by immunofluorescence chromatography. Background technique [0002] Folic acid belongs to hapten, which is a small molecular substance, which alone cannot induce immune response and has no immunogenicity. As a coenzyme of the one-carbon single-enzyme system in the biochemical reaction in the body, its main physiological functions in the human body are: 1) participate in the synthesis of purine and thymine, and further synthesize DNA and RNA; participate in amino acid metabolism; 2) participate in hemoglobin and methyl Compounds, such as epinephrine, choline, creatine, etc.; 3) Provide energy to the body, improve immunity, and help depression and anxiety; 4) Promote the maturation and growth of red blood cell nuclei, which is very important for the formation of red blood cells . [0003] Current...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/558G01N21/64
CPCG01N33/558G01N21/6486
Inventor杨帆刘亚婧曲文英杨金红朱琦琦
OwnerQINGDAO HIGHTOP BIOTECH