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Preparation process of asterias amurensis saponin with antioxidant activity

A technology of anti-oxidative activity and thorny sea turning, which can be applied in the directions of antitoxins, functions of food ingredients, medical raw materials derived from echinoderms, etc., can solve problems such as biological damage, and achieve the effect of high anti-oxidative activity

Pending Publication Date: 2022-01-18
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A certain physiological dose of active oxygen plays a very important physiological role in the organism, but excessive production of active oxygen will cause different degrees of damage to the organism

Method used

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  • Preparation process of asterias amurensis saponin with antioxidant activity
  • Preparation process of asterias amurensis saponin with antioxidant activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: A kind of preparation process of spinosa saponin with antioxidant activity, comprising the following steps:

[0029] Step 1: pretreat the spiny sea disc sample;

[0030] Step 2: Soxhlet extraction method is used to reflux the sample powder to extract starfish saponin;

[0031] Step 3: Concentrating the extract;

[0032] Step 4: Separating and extracting the saponins of P. spinosa;

[0033] Step five: performing silica gel column chromatography on the sample;

[0034] Step 6: Collecting the eluent from each part separately, concentrating and drying;

[0035] Step 7: Carry out an activity determination experiment on the saponin sample of spiny spinach;

[0036] Step 8: screening test results for antioxidant activity of saponin samples.

[0037] Further, in the first step, after drying the sea spinach sample, grind it into fine powder with a high-speed pulverizer.

[0038] Further, the extraction of crude saponin in the second step is carried out in a r...

Embodiment 2

[0043] Embodiment 2: A kind of preparation process of the saponin of spiny spinach with antioxidant activity, comprises the following steps:

[0044] Step 1: pretreat the spiny sea disc sample;

[0045] Step 2: Soxhlet extraction method is used to reflux the sample powder to extract starfish saponin;

[0046] Step 3: Concentrating the extract;

[0047] Step 4: Separating and extracting the saponins of P. spinosa;

[0048] Step five: performing silica gel column chromatography on the sample;

[0049] Step 6: Collecting the eluent from each part separately, concentrating and drying;

[0050] Step 7: Carry out an activity determination experiment on the saponin sample of spiny spinach;

[0051] Step 8: screening test results for antioxidant activity of saponin samples.

[0052] Further, in the first step, after drying the sea spinach sample, grind it into fine powder with a high-speed pulverizer.

[0053] Further, according to the extraction of the crude saponin in the seco...

Embodiment 3

[0058] Embodiment 3: A kind of preparation process of the saponin of spiny spinach with antioxidant activity, comprises the following steps:

[0059] Step 1: pretreat the spiny sea disc sample;

[0060] Step 2: Soxhlet extraction method is used to reflux the sample powder to extract starfish saponin;

[0061] Step 3: Concentrating the extract;

[0062] Step 4: Separating and extracting the saponins of P. spinosa;

[0063] Step five: performing silica gel column chromatography on the sample;

[0064] Step 6: Collecting the eluent from each part separately, concentrating and drying;

[0065] Step 7: Carry out an activity determination experiment on the saponin sample of spiny spinach;

[0066] Step 8: screening test results for antioxidant activity of saponin samples.

[0067] Further, in the first step, after drying the sea spinach sample, grind it into fine powder with a high-speed pulverizer.

[0068] Further, the extraction of the second crude saponin in the step 2 put...

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Abstract

The invention discloses a preparation process of asterias amurensis saponin with antioxidant activity. The preparation process comprises the following steps: step 1, pretreating an asterias amurensis sample; step 2, carrying out reflux extraction on the sample powder by adopting a Soxhlet extraction method to obtain starfish saponin; step 3, concentrating the extracting solution; step 4, carrying out separation and extraction on asterias amurensis saponin; step 5, carrying out silica gel column chromatography on the sample; step 6, respectively collecting the eluent of each part, concentrating and drying; step 7, carrying out activity determination experiment on the asterias amurensis saponin sample; and step 8, screening experimental results of antioxidant activity of the saponin sample. The invention belongs to the technical field of extraction, and particularly relates to a preparation process of asterias amurensis saponin with antioxidant activity, which is a method capable of utilizing wastes, has high antioxidant activity and can turn waste into wealth through the research.

Description

technical field [0001] The invention belongs to the technical field of extraction, and specifically refers to a process for preparing sea spiny saponins with antioxidant activity. Background technique [0002] Various studies have shown that in the animal kingdom, there are only saponins in the marine Echinodermata, among which sea cucumbers and starfish are more reported. Saponins have good biological activity, including antioxidant activity, antibacterial activity and antitumor activity, etc. In addition, good physiological activity is also a major feature of saponins. According to research, various tissues of starfish contain saponins. For this reason, we chose starfish, a marine organism, to study its saponin activity. Starfish also has good pharmacological value, but starfish does not have much edible value in daily life, and the research field of starfish saponin is still in its infancy in China. Cells in many organisms will continuously produce a certain concentrati...

Claims

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Application Information

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IPC IPC(8): A23L33/10A61K35/616A61P39/06
CPCA23L33/10A61K35/616A61P39/06A23V2002/00A23V2200/30A23V2250/2042
Inventor 李敏晶夏榕胡澜孙庆焦雅倩刘上
Owner DALIAN OCEAN UNIV
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