Serum-free culture medium capable of amplifying mesenchymal stem cells and preparation method thereof
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A technology of serum-free medium and mesenchymal stem cells, which is applied in the field of serum-free medium capable of expanding mesenchymal stem cells and its preparation, can solve the problems of interfering with vitamin absorption, affecting the effect of stem cell transplantation, and uneven nutrition, so as to maintain self- Renewal, increase cell adhesion performance, and inhibit canceration
Pending Publication Date: 2022-01-18
GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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However, the metformin contained in this invention will interfere with the absorption of vitamins, resulting in uneven nutrition
[0007] Therefore, there is an urgent need to develop a serum-free medium that can expand mesenchymal stem cells without animal-derived components. In addition to maintaining the ability of multidirectional differentiation of cells while increasing, ensuring that no foreign substances will cause severe immune reactions in patients, thereby affecting the effect of stem cell transplantation
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[0043] Embodiment, preparation of a serum-free medium capable of expanding mesenchymal stem cells
[0044] The serum-free medium for expanding mesenchymal stem cells uses DMEM / F12 medium as a solvent, and consists of the following components and their concentrations, see Table 1:
[0045] The composition and the concentration composition of the serum-free medium of table 1 embodiment 1~7
[0046]
[0047]
[0048] The preparation method is:
[0049] Take 100 grams of DMEM / F12 medium powder, add 900 mL of deionized water, and then add 3 g of NaHCO 3 , add 3g 4-hydroxyethylpiperazineethanesulfonic acid (Hepes), stir for 1h, adjust the pH value to 7.2, add the following ingredients: curcumin, stepherine, berberine, resveratrol, anthocyanins, Bone morphoprotein 4, β-glycerophosphate, cholesterol, isobutylmethylxanthine, EGF, biotin, aFGF, heparin sodium, reduced glutathione, transferrin, insulin, ascorbic acid, dexamethasone, interleukin 2, vitamins B12, interleukin 6, st...
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Abstract
The invention belongs to the field of detection, and particularly relates to a serum-free medium capable of amplifying mesenchymal stem cells and a preparation method thereof. The serum-free medium mainly contains curcumin, cepharanthine, berberine, resveratrol, anthocyanidin, bone morphological protein 4, beta-glycerophosphate, cholesterol, isobutyl methyl xanthine, EGF, biotin, aFGF, heparin sodium, reduced glutathione, transferrin, insulin, ascorbic acid, dexamethasone, interleukin 2, vitamin B12, interleukin 6, stem cell factors, nicotinamide, TGF-beta 3, thrombopoietin, taurine, rosiglitazone, FMS-like tyrosine kinase 3 ligand, IGF2, sodium pyruvate, GM-CSF, TPO and the like. The serum-free culture medium not only has the capacity of amplifying mesenchymal stem cells derived from fat, placenta, bone marrow and umbilical cord in vitro, but also can maintain self-renewal of the stem cells, prevent differentiation of the stem cells and inhibit canceration of the stem cells.
Description
technical field [0001] The invention belongs to the field of detection methods, and in particular relates to a serum-free medium capable of expanding mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (mesenchymaLstemceLLs, MSCs) are a kind of adult stem cells, which have many advantages such as high self-renewal, multi-directional differentiation potential, low immunogenicity, and rich sources, so they are highly valued and favored by tissue engineering and regenerative medicine. It is the most commonly used cell in regenerative medicine. [0003] MSCs are derived from bone marrow, peripheral blood, umbilical cord blood, placenta and other tissues, and are a type of adult stem cells with high self-renewal and multi-directional differentiation potential. Under different induction conditions, it can differentiate into a variety of tissue cells, and has the functions of hematopoietic support, immune regulation, tissue...
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