Serum-free culture medium capable of amplifying mesenchymal stem cells and preparation method thereof

Abstract

The invention belongs to the field of detection, and particularly relates to a serum-free medium capable of amplifying mesenchymal stem cells and a preparation method thereof. The serum-free medium mainly contains curcumin, cepharanthine, berberine, resveratrol, anthocyanidin, bone morphological protein 4, beta-glycerophosphate, cholesterol, isobutyl methyl xanthine, EGF, biotin, aFGF, heparin sodium, reduced glutathione, transferrin, insulin, ascorbic acid, dexamethasone, interleukin 2, vitamin B12, interleukin 6, stem cell factors, nicotinamide, TGF-beta 3, thrombopoietin, taurine, rosiglitazone, FMS-like tyrosine kinase 3 ligand, IGF2, sodium pyruvate, GM-CSF, TPO and the like. The serum-free culture medium not only has the capacity of amplifying mesenchymal stem cells derived from fat, placenta, bone marrow and umbilical cord in vitro, but also can maintain self-renewal of the stem cells, prevent differentiation of the stem cells and inhibit canceration of the stem cells.

Description

technical field [0001] The invention belongs to the field of detection methods, and in particular relates to a serum-free medium capable of expanding mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (mesenchymaLstemceLLs, MSCs) are a kind of adult stem cells, which have many advantages such as high self-renewal, multi-directional differentiation potential, low immunogenicity, and rich sources, so they are highly valued and favored by tissue engineering and regenerative medicine. It is the most commonly used cell in regenerative medicine. [0003] MSCs are derived from bone marrow, peripheral blood, umbilical cord blood, placenta and other tissues, and are a type of adult stem cells with high self-renewal and multi-directional differentiation potential. Under different induction conditions, it can differentiate into a variety of tissue cells, and has the functions of hematopoietic support, immune regulation, tissue...

AI Technical Summary

Problems solved by technology

However, the metformin contained in this invention will interfere with the absorption of vitamins, resulting in uneven nutrition

[0007] Therefore, there is an urgent need to develop a serum-free medium that can expand mesenchymal stem cells without animal-derived components. In addition to maintaining the ability of multidirectional differentiation of cells while increasing, ensuring that no foreign substances will cause severe immune reactions in patients, thereby affecting the effect of stem cell transplantation

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