The invention relates to sugar-free anti-ageing oral liquid and a preparing and taking method thereof. The sugar-free anti-ageing oral liquid is prepared from 12-18 parts of ginseng, 12-18 parts of fructus lycii, 8-13 parts of Chinese yam, 6-10 parts of Chinese magnoliavine fruit, 12-18 parts of radix asparagus cochinchinensis, 12-18 parts of radix ophiopogonis, 12-18 parts of radix rehmannia root, 12-18 parts of prepared rehmannia root, 18-28 parts of polygonum multiflorum, 12-18 parts of poria cocos, 12-18 parts of radix achyranthis bidentatae, 12-18 parts of radix angelica sinensis, 12-18 parts of fructus psoraleae, 12-18 parts of the bark of eucommia, 12-18 parts of morinda officinalis, 10-15 parts of rhizoma anemarrhenae, 10-15 parts of golden cypress, 5-10 parts of rhizoma curculiginis, 5-10 parts of herba epimedii, 18-28 parts of rhizoma alismatis, 12-18 parts of the root of red-rooted salvia, 12-18 parts of radix astragali, 12-18 parts of parasitic loranthus, 15-20 parts of glossy privet fruit, 16-24 parts of eclipta, 5-8 parts of cortex cinnamomi, 8-12 parts of dendrobium officinale, 0.2-3 parts of sweetening agent, 0.5-2 parts of ethylparaben and 1-3 parts of sodium benzoate.