New anticancer medicine and preparation method thereof
A new drug and carrier technology, applied in the field of medical drugs, achieves the effects of convenient preparation, inhibition of canceration and low cost
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[0025] Selected human epithelial cervical cancer cells were inoculated in 6-well plates, and the density was adjusted to 5×10 4 cells / mL, cultured in 37°C, 5% CO2 incubator for 24 hours, the cells adhere to the wall, and the area of the bottom of the plate well > 90% can be used for the experiment. Containing 5 mL of RPMI1640 culture solution of 10% calf serum in each well, set up blank group, low concentration group and high concentration group, each group has 2 wells and calculate the mean value, wherein the blank group does not add drugs, and the low concentration group adds 0.0625g / mL of the new anti-cancer drug, 0.25g / mL of the new anti-cancer drug was added to the high-concentration group, and the culture was continued for 12h, 24h, and 48h. The supernatant was removed 2h before the end of the culture, and the wavelength was measured with a photometer after shaking on an oscillator. Absorbance of each well at 570 nm, and calculate cancer cell growth inhibition rate=1-...
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