Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

LAMP-LFD-based helicobacter pylori POCT detection kit and method

A LAMP-LFD, Helicobacter pylori technology, applied in the field of medical molecular biology diagnosis, achieves high specificity, easy operation and good accuracy

Pending Publication Date: 2022-01-18
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this method has not been used for the detection of H.pylori

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • LAMP-LFD-based helicobacter pylori POCT detection kit and method
  • LAMP-LFD-based helicobacter pylori POCT detection kit and method
  • LAMP-LFD-based helicobacter pylori POCT detection kit and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0063] 1. Materials and methods

[0064] 1.1 Experimental materials

[0065] The bacterial DNA extraction kit was purchased from Dalian Bao Biological Engineering Co., Ltd., the PCR amplification kit and Bst DNA polymerase were purchased from Kangwei Century Biotechnology Co., Ltd., and the hydroxynaphthol blue indicator (hydroxy naphthol blue, HNB) was purchased from Sigman company, dNTPs mixture was purchased from Suleibao Biotechnology Co., Ltd., 5kb DNA marker was purchased from Novizan Biotechnology Co., Ltd., H.pylori (ATCC26695), Escherichia coli strains, Pseudomonas aeruginosa were preserved by our laboratory, gold The strain of Staphylococcus aureus was donated by Mr. Ni Jing, School of Public Health, Shandong First Medical University.

[0066] 1.2 Experimental method

[0067] 1.2.1 Bacterial culture and DNA extraction

[0068] Spread the H. pylori standard strain (ATCC26695) preserved in the laboratory evenly on the egg yolk agar medium, and then place it in 85% N...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an LAMP-LFD-based helicobacter pylori POCT detection kit and method, and belongs to the technical field of medical molecular biology diagnosis. Four LAMP primers and an FAM labeled probe are designed for the UreB gene of H.pylori, after a series of optimization is carried out on reaction parameters, the reaction parameters are combined with the LFD technology, the accurate and efficient H.pylori LAMP-LFD detection method is established, a reliable and effective diagnostic tool is provided for rapid and convenient on-site detection of clinical H.pylori, and therefore the detection kit has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of medical molecular biology diagnosis, and in particular relates to a LAMP-LFD-based on-site rapid detection (POCT, point-of-care testing) kit and method for Helicobacter pylori. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Helicobacter pylori (H. pylori) is the most common pathogen infecting gastric mucosa. More than 50% of the world's population is infected, and the infection rate in developing countries is generally higher than that in developed countries. Studies have shown that H.pylori can cause gastrointestinal diseases, such as gastritis, gastric and duodenal ulcers, gastric cancer, gastric m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/689C12Q1/6844C12N15/11C12R1/01
CPCC12Q1/689C12Q1/6844C12Q2531/119C12Q2563/107C12Q2563/131C12Q2565/625Y02A50/30
Inventor 赵英会刘文文吕刚王传华
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com