Human gamma delta T cell culture method
A cell culture and human technology, applied in the field of medical bioengineering, can solve the problems of difficult expansion, poor expansion ability of γδT cells, affecting the application of γδT cell therapy, etc., and achieve the effect of promoting application and solving poor expansion ability.
Pending Publication Date: 2022-01-21
杭州鑫瑞精准生物科技有限公司
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Problems solved by technology
[0003] γδT cells are immune cells that can kill cancer cells and tumor stem cells. They are used in γδT cell therapy for cancer treatment, but the expansion ability of γδT cells is poor and difficult to expand, thus affecting the application of γδT cell therapy; Therefore, a method for culturing human γδT cells is proposed to address the above problems
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[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0031] see figure 1 , a human γδT cell culture method, the culture method comprising the following steps:
[0032] S1: Blood separation, the blood is collected into heparin sodium blood collection tubes, and then centrifuged to separate the blood into 4 layers, which are composed of plasma layer, mononuclear cell layer, separation liquid layer and red blood cell layer;
[0033] S2: Inactivate the autologous plasma, collect the upper plasma with ...
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The invention belongs to the field of medical bioengineering, and particularly relates to a human gamma delta T cell culture method which comprises the following steps: S1, blood separation: collecting blood into a heparin sodium blood collection tube, and then performing centrifugal separation to divide the blood into four layers, namely a plasma layer, a mononuclear cell layer, a separation liquid layer and a red blood cell layer; S2, inactivating autologous plasma: collecting upper-layer plasma by using a transfer pipette, heating the plasma, then carrying out centrifugal separation, then collecting supernate into a new centrifugal tube by using a suction tube, and carrying out low-temperature storage; and S3, preparing PBMC: collecting the second layer of mononuclear cells are collected into a new centrifugal tubule through a transfer pipette. By means of the scheme, in-vitro proliferation culture of human gamma delta T cells is achieved, the problems that the gamma delta T cells are poor in proliferation capacity and difficult to amplify are solved, and therefore application of gamma delta T cell therapy is promoted.
Description
technical field [0001] The invention relates to the field of medical bioengineering, in particular to a method for culturing human γδT cells. Background technique [0002] Lymactin-γδT antibody is used to specifically activate γδT cells. It is an exogenous non-peptide antigen. It is a colorless clear liquid after preparation. Lymactin-γδT antibody stimulates and activates Vγ9Vδ2T cells, and up-regulates the expression of perforin and granzyme in γδT cells. Activates the cytotoxic function of γδ T cells. [0003] γδT cells are immune cells that can kill cancer cells and tumor stem cells. They are used in γδT cell therapy for cancer treatment, but the expansion ability of γδT cells is poor and difficult to expand, thus affecting the application of γδT cell therapy; Therefore, a method for culturing human γδT cells is proposed to address the above problems. Contents of the invention [0004] In order to make up for the deficiencies of the prior art, at least one technical p...
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IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2509/00C12N2501/2302
Inventor 章万古
Owner 杭州鑫瑞精准生物科技有限公司