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Preparation method and application of pawpaw biological enzyme

A technology of biological enzymes and papaya, applied in biochemical equipment and methods, botany equipment and methods, chemicals for biological control, etc.

Pending Publication Date: 2022-02-11
GUANGZHOU CITY POLYTECHNIC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researchers compared the sequence of the new coronavirus with the SARS coronavirus and found that the two are very similar; by comparing the S protein of the 2019-nCoV with the S protein of the SARS coronavirus, and then building a model by computer, it was found that although the interacting Four of the five amino acids have been mutated, but there is still the possibility of interaction between the S protein of the new coronavirus and the ACEII protein of the human body as a whole

Method used

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  • Preparation method and application of pawpaw biological enzyme
  • Preparation method and application of pawpaw biological enzyme
  • Preparation method and application of pawpaw biological enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] See figure 1 In one embodiment of the present invention, a method of preparing a papaya bioassase and the production process thereof use of freezing extraction, ultrafiltration and narrow separation, equidistor purification, enzymatic protection and scientific compatibility technology. Papaya Pouga Paste Solly extract, the extracted slurry is subjected to ultrafiltration and narrow separation after microfiltration, and then purified by the slurry after ultrafiltration and narrow separation, and then protects it into a solid Get it into the pulverizer, it is sold after the test, the papaya lysin semi-finished product is obtained, and the papaya lysin semi-finished product is sold after the factory. 10%, pineapple extract 10%, pineapple extract, 2%, other 78%, according to the biological disinfectant formulation design, weighted to mix the mixed device, mixed, mixed products After homogeneous dissolution And the test is qualified.

[0032] Existing lysozyme industrial product...

Embodiment 2

[0034] See figure 2 In the present invention, the present invention is provided: a method for preparing papaya bioase and its application of production process, papaya lysozy production process:

[0035] S1, picking papaya cultivated through bio-regulatory techniques, making papaya into papaya, spare;

[0036] S2, slowly stir the papaya pollus, add Ca and L-cysteine ​​protective agent mixing to high viable parquet pulp, spare;

[0037] S3, the high viable papaya pulp is fed in the ice space of -18 ° C for 60 days, and then take out and thaw from the cold storage after 60 days;

[0038]S4, adding the thawed high vibrant pumpkin pulp to the stirring device, the stirring device is arranged to 3500 rpm, at high speed for 15 minutes;

[0039] S5, the high vibrant papaya pulp after stirring was added with pure water in a ratio of 1: 3, and the stirring device was set to 10 Hz for stirring for 3 hours;

[0040] S6, the slurry after stirring is separated from the horizontal helix device, ...

Embodiment 3

[0046] See image 3 One embodiment of the present invention: a method for preparing papaya bioassase and its application production process, new biomease disinfectant product production process:

[0047] S1, put the papaya lysin semi-finished product, pineapple extract, and theureidase in accordance with the biological disinfectant formulation, it is mixed, and the mixture is added to the mixture after weighing.

[0048] S2, the mixture is added to the pulverization device for pulverization, pulverizing three-dimensional mixing, and dissolving the solvent homogenesis, resulting in a formulation;

[0049] S3, the obtained formulation is detected, and the package is packaged after passing the qualified.

[0050] From the ultrastructure of bacteria, the expression of viration factors, the formation of biofilms and the angle of protein groups, the antibacterial effect and the mechanism of antibacterial effects of Papaya. Papaya extracted lysozymes can decompose the glycoprotein of the ...

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Abstract

The invention discloses a preparation method and application of a pawpaw bio-enzyme. The production process adopts freeze-dissolving extraction, ultrafiltration narrow separation, isoelectric point purification, enzyme activity protection and scientific compatibility technology for production, wherein freeze-dissolving extraction is performed on papaya pulp, the extracted pulp is subjected to microfiltration and then is subjected to ultrafiltration narrow separation, isoelectric point purification is performed on the pulp subjected to ultrafiltration narrow separation, enzyme activity protection is performed, freeze-drying is performed to form a solid, the solid is fed into a crushing device for crushing, inspecting is performed to obtain a papaya lysozyme semi-finished product, a pineapple extract and lysostaphin are added into the papaya lysozyme semi-finished product, a ratio is designed according to a formula of a biological disinfectant, weighing, mixing, homogenizing and dissolving are sequentially performed, detection is performed to achieve a qualified standard, and sub-packaging is performed to obtain the novel biological enzyme disinfectant, wherein the in-vitro sterilization effective rate of the novel biological enzyme disinfectant product reaches 90% or above, and the novel bio-enzyme disinfectant product has the effects of disinfection, sterilization and the like, and has the advantage of food-grade safety.

Description

Technical field [0001] The present invention relates to the field of production technology of biological enzyme preparation, and is specifically a preparation method for papaya bioase and its application. Background technique [0002] The study found that the SARS virus is an intrusion of the human body through the s protein of the viral envelope surface with the human vascular tension transferase II (ACEII) of the human body. The researchers compare the sequence of new coronaviruses with SARS coronaviruses, found that both are very similar; through comparison 2019-NCOV s proteins with S proteins of SARS coronavirus, then establish models through computer, found although interaction There are four mutations in the five amino acids, but the S protein of new coronaviruses and the human ACEI protein is still interacting. This shows that the new coronavirus is likely to complete infection through the molecular mechanism of the S protein interact with human ACEII. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/36A01N65/40A01N63/50A01P1/00
CPCC12N9/2462C12Y302/01017A01N65/00A01N65/40A01N63/50
Inventor 刘慧娟吴新良黎海彬
Owner GUANGZHOU CITY POLYTECHNIC