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Multifunctional American ginseng hydrolytic peptide as well as preparation method and application thereof

A technology of American ginseng and hydrolyzed peptides, which is applied in the field of medical functional product processing, can solve the problems of complex composition, unfavorable promotion and application, complex preparation process, etc., and achieve the effects of improving scientific and technological content, strong blood pressure and blood sugar control ability, and simple operation.

Pending Publication Date: 2022-02-11
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The preparation of American ginseng polypeptide in the prior art mainly uses organic solvents or expensive extraction equipment. The preparation process is complicated and the cost is high, which is not conducive to the promotion and application of the market. The purity of polypeptide products is low, how to simplify the extraction process and effectively improve the purity of American ginseng polypeptide is a technical problem faced by those skilled in the art.

Method used

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  • Multifunctional American ginseng hydrolytic peptide as well as preparation method and application thereof
  • Multifunctional American ginseng hydrolytic peptide as well as preparation method and application thereof
  • Multifunctional American ginseng hydrolytic peptide as well as preparation method and application thereof

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Effect test

Embodiment 1

[0059] A preparation method of multifunctional American ginseng hydrolyzed peptide, the specific steps are as follows:

[0060] (1) After crushing 1kg American ginseng, add 0.004mol / L 1-butyl-3-methylimidazolium tetrafluoroborate solution at a mass volume ratio of g / mL 1:20, extract at 55°C for 2 hours, and obtain American ginseng crude protein Extraction solution;

[0061] (2) Take American ginseng crude protein extract, add dipotassium hydrogen phosphate according to the volume to mass ratio of mL / g 12:0.8, 70KHz ultrasonic vibration for 1h, and centrifuge at 4000r / min for 10min; separate the liquid, remove the lower liquid, and the supernatant Add polyethylene glycol 600 with a final concentration of 15% to the solution, let it stand at 4°C for 24 hours, centrifuge at 4000r / min for 20 minutes, and collect the precipitate to obtain the American ginseng protein extract.

[0062] (3) The protein extract is dissolved in 12 times the amount of water, the amount of pepsin added ...

Embodiment 2

[0064] A preparation method of multifunctional American ginseng hydrolyzed peptide, the specific steps are as follows:

[0065] (1) After crushing 1kg American ginseng, add 0.01mol / L 1-butyl-3-methylimidazolium tetrafluoroborate solution according to the mass volume ratio g / mL 1:35, and extract at 70°C for 0.5h to obtain crude American ginseng protein extract;

[0066] (2) Take the American ginseng crude protein extract, add dipotassium hydrogen phosphate according to the volume to mass ratio of mL / g 15:0.8, oscillate ultrasonically at 100KHz for 0.5h, and centrifuge at 5000r / min for 5min; separate the liquid, remove the liquid in the upper layer, and Add polyethylene glycol 200 with a final concentration of 20% to the supernatant, let it stand at 4°C for 48 hours, centrifuge at 5000r / min for 15 minutes, and collect the precipitate to obtain the American ginseng protein extract.

[0067] (3) Dissolve the protein extract with 10 times the amount of water, add 20 mg / mL of pepsi...

Embodiment 3

[0069] A preparation method of multifunctional American ginseng hydrolyzed peptide, the specific steps are as follows:

[0070] (1) After crushing 1kg American ginseng, add 0.001mol / L 1-butyl-3-methylimidazolium tetrafluoroborate solution at a mass-volume ratio of g / mL 1:10, and extract at 40°C for 4 hours to obtain American ginseng crude protein Extraction solution;

[0071] (2) Take American ginseng crude protein extract, add dipotassium hydrogen phosphate according to the volume to mass ratio of mL / g 5:0.8, oscillate ultrasonically at 40KHz for 2h, and centrifuge at 3000r / min for 15min; separate the liquid, remove the lower liquid, and supernatant Add polyethylene glycol 1000 with a final concentration of 5% to the solution, let it stand at 4°C for 12 hours, centrifuge at 3000r / min for 30 minutes, collect the precipitate to obtain the American ginseng protein extract.

[0072] (3) Dissolve the protein extract in 15 times the amount of water, add 100mg / mL pepsin for primary...

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Abstract

The invention relates to a multifunctional American ginseng hydrolytic peptide as well as a preparation method and application thereof; the content of active peptide in the American ginseng hydrolytic peptide provided by the invention is up to 85% or above, and the molecular weight range of the active peptide is less than 10kD; The invention further provides a preparation method of the American ginseng hydrolyzed peptide. The method comprises the step of extracting the American ginseng hydrolytic peptide by utilizing an ionic liquid solution and a pepsin-trypsin two-stage digestion process. The method is green and safe in technological process, simple to operate and convenient for large-scale production. The American ginseng hydrolytic peptide provided by the invention is remarkable in functional effect, such as the functions of regulating immunity, reducing blood pressure, reducing blood sugar, resisting inflammation, resisting oxidation and the like; besides, the pharmacodynamic experiments show that a drug combination mode has a synergistic effect on part of symptoms.

Description

technical field [0001] The invention relates to a multifunctional American ginseng hydrolyzed peptide, a preparation method and application thereof, and belongs to the field of processing medical functional products. Background technique [0002] Organic solvents are often used in the extraction and separation of natural active ingredients. They not only have strong volatility or toxicity, but also cause environmental pollution during the production process. Ionic liquids are liquid substances composed of larger, asymmetrically structured organic cations and smaller inorganic / organic anions. Compared with traditional solvents, ionic liquids have the characteristics of good thermal stability, non-volatility, safety and environmental protection, and easy reuse. And because of its high selectivity to target substances, it can effectively extract active ingredients in natural products, and has become a new type of green solvent in the field of purification and separation. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30A61K38/01A61K8/64A61P3/10A61P9/12A61P29/00A61P37/02A61P39/06A61Q19/00A61Q19/08A23L33/18A61K31/7036
CPCC12P21/06C07K1/36C07K1/34C07K1/30A61K38/011A61K31/7036A61P37/02A61P9/12A61P3/10A61P29/00A61P39/06A61K8/645A61Q19/08A61Q19/00A23L33/18A61K2800/805A61K2800/82A61K2800/522A23V2002/00A61K2300/00A23V2200/30A23V2200/324A23V2200/328A23V2200/326A23V2250/55Y02P20/54
Inventor 张轩铭李晓彬张姗姗何秋霞夏青王利振侯海荣刘可春陈锡强盛文龙王西新
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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