Molecular marker cm17-16 for rapidly detecting pumpkin naked kernel traits and application thereof

Abstract

The invention belongs to the technical field of pumpkin molecular breeding, and particularly relates to a molecular marker cm17-16 for rapidly detecting pumpkin naked kernel traits and application thereof. The molecular marker cm17-16 is located on the No.17 chromosome of a pumpkin genome; and during detection, DNA of a pumpkin to be identified is firstly extracted, then the extracted DNA is used as a template, a primer pair for amplifying the molecular marker cm17-16 is used for performing PCR amplification, then a PCR product is subjected to electrophoresis, dyeing and developing, and finally whether the pumpkin to be identified is a seed coat pumpkin or a nude pumpkin is analyzed by reading bands. The InDel molecular marker is used for identifying the pumpkin nude or seed coat character for the first time, and meanwhile, the invention discloses a primer sequence of the InDel molecular marker on the No.17 chromosome linked with the pumpkin nude character; and the molecular marker cm17-16 disclosed by the invention can be used for rapidly and accurately identifying pumpkin nude kernel or seed coat characters in the early breeding stage, and provides powerful support for further accelerating the utilization of pumpkin and important agronomic character linkage genes and pumpkin molecular genetic breeding.

Description

Technical field [0001] The present invention belongs to the field of pumpkin molecular breeding technology, and more particularly to a molecular mark CM17-16 for rapid detection of pumpkin naked kernels and its applications. Background technique [0002] Cucurbita Moschata Duchesne belongs to the one-year herbal plant, with bamboo shoots, and the zucchini is the main three cultivars for pumpkins. The three are not only developed in the production of meat, but also in the production and utilization of seeds. In terms of seedplanted seeds in pumpkins such as zucchini, bamboo shoots, pumpkins and other pumpkins, my country's pumpkin seeds is based on 70% of the world market share. Domestic pumpsence seeds (including seeds) consumption is about 200,000 tons, and the demand for pumpkin seeds at home and abroad has grown by 17%. As one of the important functional foods with pumpkins, it is important to promote industries to high-end development. [0003] Pumpkin seed is sweet, tempered...

AI Technical Summary

Problems solved by technology

At present, InDel markers have been widely used in crops such as corn, rice, Chinese cabbage, cucumber, etc., but there are no reports on the use of InDel markers to detect the traits of pumpkin naked kernels

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