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Novel coronavirus nucleic acid detection kit

A technology for detection kits and coronaviruses, which can be used in the detection/testing of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., and can solve the problems of detection kit sensitivity and specificity to be improved, short development cycle, etc. , to achieve real-time observation of data changes, avoid contamination of samples, and ensure safety

Active Publication Date: 2022-02-11
HANGZHOU ANYU TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current novel coronavirus antibody detection kit also needs to be improved due to the short development cycle, and the sensitivity and specificity of the detection kit

Method used

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  • Novel coronavirus nucleic acid detection kit
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  • Novel coronavirus nucleic acid detection kit

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Experimental program
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Effect test

Embodiment 1

[0040] Development of Novel Coronavirus Nucleic Acid Detection Kit

[0041] 1. Design of primers

[0042]According to the new coronavirus diagnosis and treatment guidelines, in order to ensure the detection of the new coronavirus and avoid false positives or false negatives, the conserved regions of the new coronavirus ORF1ab gene (Gene ID: 43740578) and S gene (Gene ID: 43740568) were selected as targets Click to check.

[0043] Refer to the general technical regulations of the cross-primer constant temperature amplification detection method, and design primers according to the following primer design requirements:

[0044] (1) The distance between the stripping primers is 150-200 bp, the length of the stripping primers is 17-20 bp, and the temperature is between 48-58 °C;

[0045] (2) The distance between the cross-amplification primers is between 45 and 100 bp, the length of the cross-amplification primers is between 34 and 42 bp, and the temperature of a single fragment ...

Embodiment 2

[0058] 1. Design of primers

[0059] With embodiment 1.

[0060] 2. Composition of the Novel Coronavirus Nucleic Acid Detection Kit

[0061] The composition and concentration of the novel coronavirus nucleic acid detection kit are shown in Table 4 and Table 5, respectively.

[0062] Table 4 Composition of the Novel Coronavirus Nucleic Acid Detection Kit

[0063] component name Capacity (μL) Element first container The reaction solution 800 dNTPs, 10× buffer, cyclohexanol succinate second container Detection solution 50 Primer, SYBR Green I third container Enzyme solution 200 Reverse transcriptase, Bst DNA polymerase, ALV reverse transcriptase, RNase inhibitor fourth container MgCl 2

[0064] Table 5 Concentration of each component of the novel coronavirus nucleic acid detection kit

[0065] Element concentration dATP 500nM dTTP 500nM dCTP 500nM dGTP 500nM cyclohexyl succinate ...

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Abstract

The invention discloses a novel coronavirus nucleic acid detection kit, and belongs to the technical field of biological detection. According to the invention, the novel coronavirus is detected by using a real-time fluorescent quantitative cross primer isothermal amplification technology, and two groups of primers suitable for the technology are designed. The two groups of primers are respectively used for detecting an ORF1ab gene in novel coronavirus genome and an S gene in novel coronavirus genome. The novel coronavirus nucleic acid detection kit disclosed by the invention is strong in specificity and high in sensitivity, not only can qualitatively and quantitatively detect a sample, but also can observe the change of data in real time, is beneficial to comprehensive collection of sample data, and is convenient for next-step research. Therefore, the method is not only suitable for on-site rapid detection, but also suitable for detection aiming at scientific research.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a novel coronavirus nucleic acid detection kit. Background technique [0002] Coronavirus (Coronavirus, CoV) is a class of RNA viruses with an envelope and a linear single-stranded positive strand genome. It is a large class of viruses that exist widely in nature. Coronaviruses are divided into 4 genera of α, β, γ and δ, among which α and β coronaviruses can infect mammals, while γ and δ coronaviruses mainly infect birds. It has been found that 6 coronaviruses can infect humans: αcoronavirus (HCoV-229E, HCoV-NL63) and βcoronavirus (HCoV-HKU1, HCoV-OC43, MERS-CoV, SARS-CoV). Cold to severe lung infection. Novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) is a betacoronavirus that has not been found in humans before. It is highly contagious in the crowd, mainly through respiratory droplets, Transmission by respiratory secretions and dir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11
CPCC12Q1/701C12Q1/6844C12Q2600/166C12Q2531/119C12Q2537/1376C12Q2563/107C12Q2527/127C12Q2521/107C12Q2545/113Y02A50/30
Inventor 冯瑞娟章贤骏李海锋陈倩倩
Owner HANGZHOU ANYU TECH CO LTD
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