Nano-carrier for treatment of tumor through combination of chemotherapy and radiotherapy as well as preparation method and application of nano-carrier

A combination therapy and nanocarrier technology, applied in the field of biomedical nanomaterials, can solve the problems of short half-life, aggravate systemic side effects, etc., and achieve good therapeutic effect, good biosafety, and enhanced biocompatibility.

Pending Publication Date: 2022-02-18
NANJING UNIV OF TECH
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Problems solved by technology

On the other hand, small molecule chemotherapeutic drugs have a short half-life in the body and are easy to be cleared quickly. In order to ensure the che

Method used

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  • Nano-carrier for treatment of tumor through combination of chemotherapy and radiotherapy as well as preparation method and application of nano-carrier
  • Nano-carrier for treatment of tumor through combination of chemotherapy and radiotherapy as well as preparation method and application of nano-carrier
  • Nano-carrier for treatment of tumor through combination of chemotherapy and radiotherapy as well as preparation method and application of nano-carrier

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[0037] For this reason, the present invention proposes a kind of preparation method that is used for the combination treatment nanocarrier of tumor chemotherapy and radiotherapy, comprises the following steps:

[0038] Step 1, select lysine as a raw material, use ethylenediamine as the core, and synthesize G1-G3 generation peptide dendrimers by a divergent method;

[0039] Step 2, modifying mPEG on the surface of G1-G3 peptide dendrimers as an acid-sensitive bond, and synthesizing Boc-Hyd-G1-G3-mPEG (Boc-Hyd-PP) nanoparticles as a carrier;

[0040] Step 3, linking the chemotherapeutic drug DOX with the obtained nanoparticles through a chemical bond to obtain DOX-Hyd-PP;

[0041] Step 4. Using the in-situ recombination method, the gold ions coated in the cavity of the nanoparticles are in-situ reduced, and the gold ions are reduced to nano-silver and gold loaded therein to obtain double drug-loaded nanoparticles DOX-Hyd-PP@AuNPs.

[0042] Wherein, the concrete processing of de...

Example Embodiment

[0060] Example 1

[0061] In order to determine the DOX release of drug-loaded materials in vitro, we simulated the in vivo conditions for drug release experiments. Weigh 4mg of DOX-Hyd-PP and dissolve it in 1mL of PBS buffer with different pH (pH5.4, 6.8, 7.4), transfer the liquid into a dialysis bag with a cut-off flow of 1000, clamp the dialysis bag and put it into 25mL of PBS buffer buffer (pH5.4, 6.8, 7.4) in a centrifuge tube. Put the centrifuge tube into a constant temperature shaker at 37°C and 120r / min to start timing. 1 mL was taken out at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 18, 24, 36, 48, 60, 72, 84, and 96 h and supplemented with PBS buffer of corresponding pH. The fluorescence absorption value of DOX at 480nm was measured with an ultraviolet spectrophotometer, and the amount of DOX released at different time points was calculated according to the DOX concentration-absorption intensity standard curve, and the cumulative release amount was calculated by taking the...

Example Embodiment

[0063] Example 2

[0064] In order to track the phagocytosis of nanogels in cells, 4T1 cells were divided into 4 × 10 4 Cells were seeded at a confocal laser dish and incubated for 24 h. After the cells adhered to the wall, the medium was discarded, and fresh medium of DOX.HCl and DOX-Hyd-PP@AuNPs (DOX content of 5 μg / mL) was added to incubate for 2 h and 6 h, respectively. After the incubation, the medium was removed, washed three times with PBS, fixed with 4% paraformaldehyde for 15 min, and washed three times with PBS. Add ready-to-use DAPI staining agent for staining for 8 minutes, wash with PBS three times, add anti-fluorescence quenching mounting solution to seal the slide, and use laser confocal to observe the fluorescence intensity in the cells.

[0065] Then 4T1 cells were divided into 4 × 10 per well 5 Each cell density was seeded in a 6-well plate, and each group was set up with 3 replicate wells, and 2 mL of complete medium containing 10% fetal bovine serum was ...

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Abstract

The invention provides a nano-carrier for treatment of tumor through a combination of chemotherapy and radiotherapy, and a preparation method and application of the nano-carrier. The nano-carrier is composed of amphiphilic peptide dendrimers; the hydrophilic end of the nano-carrier is composed of mPEG, and the hydrophobic end of the nano-carrier is composed of the chemotherapy drug doxorubicin (DOX); and the nano-carrier comprises peptide dendrimer nano-particles serving as a carrier and a radiotherapy sensitizer, namely gold nano-particles (AuNPs) loaded in inner cavities of the peptide dendrimer nano-particles. The nanoparticle drug carrier prepared on the basis of the method in the invention and used for chemotherapy-radiotherapy combined treatment has a particle size of 90-180 nm, and can enter tumor cells through endocytosis, so rapid metabolism of small molecule chemotherapy drugs in in-vivo circulation is effectively reduced; and the AuNPs can effectively enhance the level of ROS (reactive oxygen species) in cells under X-ray irradiation, so mitochondrial potential is unbalanced, a cell cycle is influenced, and then, the AuNPs are cooperated with chemotherapy drugs to induce cell apoptosis to inhibit the growth of cancer cells, thereby achieving the aim of inhibiting tumors.

Description

technical field [0001] The invention relates to the technical field of biomedical nanomaterials, in particular to a nanocarrier used in the combined treatment of tumor chemotherapy and radiotherapy, a preparation method of the nanocarrier, and an application of the nanocarrier in the combined treatment of chemotherapy and radiotherapy. Background technique [0002] Cancer is one of the major diseases that endanger human life and health. According to the World Health Organization, cancer cases and deaths are increasing rapidly, and cancer is expected to become the leading cause of death worldwide in the 21st century. Therefore, it is urgent to develop highly effective anticancer strategies. At present, chemotherapy is widely used in clinical treatment, but the non-selective characteristics of small molecule chemotherapy drugs make them kill normal tissues and cells, resulting in very obvious toxic side effects, such as the broad-spectrum anticancer drug doxorubicin (DOX), w...

Claims

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Application Information

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IPC IPC(8): A61K47/64A61K31/704A61K33/242A61P35/00B82Y5/00B82Y40/00
CPCA61K47/64A61K31/704A61K33/242A61P35/00B82Y5/00B82Y40/00A61K2300/00
Inventor 张亚男王朋飞杨昕刚郭苗胡玉冰
Owner NANJING UNIV OF TECH
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