System device, method and application for quantitative sample injection of chip detection sample
A system device and chip detection technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problems of new reaction groups, dependence, high cost, etc., to achieve convenient and flexible operation, reduce dependence, Realize the effect of automation
Active Publication Date: 2022-03-01
江苏液滴逻辑生物技术有限公司
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- Abstract
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- Claims
- Application Information
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Problems solved by technology
[0006] Once the traditional PCR reaction is started, it is impossible to add a reaction group in the middle, and the manual operation is time-consuming and labor-intensive. The digital microfluidic chip adopts the principle of electrowetting technology, which regulates the surface energy of solid and liquid through potential, and utilizes the difference in surface energy. The equilibrium state drives the liquid to move, so as to achieve precise control of the micro-liquid, which greatly depends on the syringe pump and the cost is high
Method used
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Embodiment 1
[0099] In this embodiment, a system device for quantitative sample injection of chip detection samples is provided, such as figure 1 As shown, the system device includes a sample quantification component 1 and a liquid injection chamber 2, such as figure 2 As shown, the sample quantification component 1 includes a body 3, a quantification chamber 5 is arranged in the body 3, a groove is provided on the outer wall of the body 3, and an "O"-shaped sealing ring 4 is arranged in the groove. The sample quantification component 1 also includes a base for fixing the body 3. During the sample injection process, the edge of the base and the inner wall of the housing 7 are in an interference fit to achieve sealing. The side surface of the base close to the body 3 is also provided with four guides. 6. There is a gap between the body 3 and the guide 6.
[0100] like image 3 As shown, the liquid injection chamber 2 includes an open housing 7, the housing 7 is provided with a partition ...
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The invention provides a system device and method for quantitative sample injection of a chip detection sample and application, the system device is used for injecting the quantitative detection sample into a gap cavity of a chip, the system device comprises a sample quantifying assembly and a liquid injection chamber, the sample quantifying assembly comprises a body, a quantifying chamber is arranged in the body, and the liquid injection chamber is arranged in the body. The liquid injection cavity comprises an open shell, a partition plate is arranged in the shell and divides the shell into a first cavity and a second cavity, a detection sample is injected into the first cavity, the sample quantifying assembly is arranged in the liquid injection cavity and is continuously pressed downwards, and the body extends into the first cavity for quantitative sample injection. The detection sample is quantitatively injected without an additional quantitative tool, so that the automation of chip sample injection is realized, the dependence on the additional quantitative tool is reduced, and the operation is more convenient and flexible.
Description
technical field [0001] The invention belongs to the technical field of microfluidic chips, and relates to quantitative sample injection in a chip, in particular to a system device, method and application for quantitative sample injection of a chip detection sample. Background technique [0002] During the polymerase chain reaction (PCR) reaction process, the test sample needs to be added by the test personnel when using the chip after the sample is collected and processed. Usually the test sample is collected in the collection tube and needs to be injected into the sample in the digital microfluidic chip. The amount is certain. If liquid quantitative tools such as pipettes and droppers are used to quantitatively remove the liquid from the collection tube and then inject it into the sample inlet of the chip, the user's operation steps will be increased, and the usage scenarios of the digital microfluidic chip will be greatly limited. [0003] CN109652298A discloses a droplet ...
Claims
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CPCB01L3/502707
Inventor 杜佩苏阳张研
Owner 江苏液滴逻辑生物技术有限公司