Integrated nucleic acid detection chip and method based on CRISPR technology

Abstract

According to the integrated nucleic acid detection chip and method based on the CRISPR technology, an isothermal amplification technology and a CRISPR molecular diagnosis technology can be carried out in one device at the same time, the problem that the two technologies are incompatible is solved through integrated detection, and through simple manual operation, the detection efficiency is greatly improved. A user can realize high-sensitivity nucleic acid detection, so that the support of large-scale instruments and equipment is avoided, and the use cost is greatly reduced. The device is convenient to carry, provides a good platform for separation of a CRISPR molecular diagnosis technology from a central laboratory, is convenient to operate, accelerates the nucleic acid detection speed, saves a part of medical cost, and solves the problems of slow detection result, high cost and incapability of realizing detection integration in the prior art.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an integrated nucleic acid detection chip and method based on CRISPR technology. Background technique [0002] Nucleic acid detection is the gold standard for identifying biological species and an important means for the detection and identification of viruses, bacteria and other microorganisms. It has important applications in the fields of disease diagnosis, epidemic prevention and control, and health monitoring. At present, the commonly used nucleic acid detection is based on fluorescent quantitative polymerase chain reaction (qPCR) to amplify the DNA of pathogens. However, qPCR has a long detection cycle and is equipped with expensive modules such as precise temperature control and optical detectors. The pollution control is stricter, resulting in cost high. RNA detection based on qPCR is limited to the central laboratory, which has great limitations on rapid on-site detection. I...

AI Technical Summary

Problems solved by technology

However, the overall structure of the chip is complex, and it needs to cooperate with a rotating turntable and a common thermal block to control the flow and reaction of the reaction solution. The cost is high, the steps are cumbersome, and it is not easy to carry. Moreover, the reversible rotary valve needs to be rotated manually, and it is difficult to be accurate in the actual use process. operate

[0005] At present, CRISPR nucleic acid detection technology is mainly concentrated in the laboratory, and there are few existing platforms that can take into account the characteristics of accuracy, low cost, and portability to realize CRISPR technology on-site detection

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