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Method for producing sterile fairy shrimps and application thereof

A technology of artemia and sterile water, which can be applied in the fields of application, climate change adaptation, fish farming, etc., and can solve problems such as not found

Pending Publication Date: 2022-03-22
重庆绵凯生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the published results show that sterile zebrafish can only be cultured to the 7th day after fertilization, which may be due to the lack of suitable sterile feed to support its survival to adult fish.

Method used

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  • Method for producing sterile fairy shrimps and application thereof
  • Method for producing sterile fairy shrimps and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Such as figure 1 Shown, the production method of sterile artemia comprises the steps:

[0053] 1. Prepare the NaClO solution, eggs, pump head, hose, 0.22μm filter membrane, autoclaved and air-dried Erlenmeyer flask, 150mm×90mm crystallization dish, 2.25% saline and medical Absorbent cotton for use.

[0054] 2. Place the pump head and hose connection in a crystallization dish, add 300mL (generally submerged in the pump head) NaClO solution with a concentration of 6g / L into the crystallization dish, so that the pump head and hose are all submerged below the liquid surface , use tweezers to assist in dislodging air from the hose.

[0055] 3. Rinse 4g of eggs with NaClO solution with a concentration of 3.6g / L for 3 minutes, and shake gently at intervals to ensure that each egg can fully contact with the bleach. Be careful not to have air bubbles in the solution.

[0056] 4. Use a sterilizing filter to filter the rinsed eggs, and rinse with sterilized saline for 1 minute...

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Abstract

The invention belongs to the technical field of aquaculture, and particularly discloses a method for producing sterile fairy shrimps and application of the sterile fairy shrimps. The method comprises the following steps: (1) bleaching fairy shrimp eggs with a bleaching agent; (2) filtering the eggs bleached in the step (1) by using a filter screen, and washing by using sterile water to remove the bleaching agent on the surfaces of egg shells; and (3) culturing the eggs obtained in the step (2) in saline water, hatching the eggs, and then separating the hatched fairy shrimps from egg shells to obtain the sterile fairy shrimps. The effective sterile fairy shrimp production method is established, safe and reliable feed is provided for sterile fishes, hope is provided for establishing an adult sterile fish model, and convenient materials are provided for studying interaction and multiple biology between microorganisms and fishes by using the sterile fishes; the method is of great significance to further research on the internal mechanism of the relationship between microorganisms and vertebrate hosts thereof.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for producing sterile artemia and its application. Background technique [0002] Germ-free animals can be used to study how microorganisms colonize or how microbial metabolites affect host biological processes, including gene expression, development, physiology, immunity and lifespan. This can be accomplished by exposing germ-free animals to individual species, specific microorganisms or products at specific time points and then analyzing the host's response. Host functions and microbial gene products can be detected by performing genetic manipulations in the corresponding strains. At the same time, germ-free animals are a very good platform to study the interaction between microorganisms and microorganisms in the physiological environment of living hosts. They can be colonized in sterile hosts with certain microbial species or genotype combinations, and then Compe...

Claims

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Application Information

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IPC IPC(8): A01K61/59A23K50/80A23K10/22
CPCA01K61/59A01K61/17Y02A40/81
Inventor 裴得胜文萍萍
Owner 重庆绵凯生物技术研究院有限公司
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