Method for preparing neurodevelopmental defect rat model through 3-MA treatment in weaning period of rats

Abstract

The invention provides a method for preparing a neurodevelopmental defect rat model through treatment with 3-MA in a weaning period of rats, which comprises the following steps: performing intraperitoneal injection of an autophagy inhibitor 3-MA on the rats in a normal weaning period continuously for 7 days to prepare a rat model for inhibiting autophagy generation, and detecting autophagy generation through immunoblotting. Then verifying social barriers through a three-box social experiment, verifying whether the anxiety level of the rat model is increased through an open field experiment, an elevated cross labyrinth experiment and a black and white box experiment, verifying whether the rat model has memory impairment through a Y labyrinth experiment and a passive avoidance experiment, and performing Golgi body staining on the rat model and a control group; and the neuron and synaptic morphology of the hippocampus and the frontal lobe Through multiple experiments, it is found that in the weaning period, 3-MA treatment for preparing the rat model with the neurodevelopment defect has the advantages of being low in cost, short in modeling period, more obvious in model behavior abnormality and better in stability, and can be used for research of diseases such as schizophrenia and autism and research and development of new drugs.

Description

technical field [0001] The invention belongs to the technical field of psychiatric disease research, and in particular relates to a method for preparing a neurodevelopmental defect rat model by treating rats with 3-MA during the weaning period. Background technique [0002] A large number of studies have shown that maternal infection during pregnancy is a high-risk factor for mental disorders and neurological diseases, and the risk of their offspring suffering from schizophrenia, autism, bipolar disorder, and major depression is significantly increased. Based on epidemiological findings, the most commonly used model of neurodevelopmental disorders in basic research on mental illness is the rat model of maternal infection during pregnancy. However, the modeling cycle of this model is long, the success rate of the offspring model is low, and the model is unstable. In view of the poor effect of this model, researchers at home and abroad improved on the basis of this model and ...

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Our research group has also tried the "two-strike model" model many times, but the effect is not good, and the p

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