3D stacked multi-stage inertial microfluidic sorting chip and preparation method thereof
A stacking, microfluidic technology, applied in the field of microfluidic technology and cell sorting technology, achieves the effect of simple preparation, high-efficiency sorting, automation of speed reduction, and improved integration.
Active Publication Date: 2022-04-01
HANGZHOU DIANZI UNIV
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[0005] The purpose of the present invention is to provide a 3D stacked multi-stage inertial microfluidic sorting chip, which aims to solve the deficiencies of the existing inertial microfluidic chips. Provide solutions for portable and fast medical diagnosis
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[0034] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings.
[0035] Such as figure 1 As shown, the 3D stacked multi-stage inertial microfluidic sorting chip of the present invention is formed by stacking 6-layer chip units; the 6-layer chip units are respectively the first chip unit A, the second chip unit B, and the third chip unit C, the fourth chip unit D, the fifth chip unit E and the sixth chip unit F. The first chip unit A adopts a trapezoidal cross-section spiral pipe for preliminary cell sorting; the second chip unit B adopts a symmetrical rectangular cross-section serpentine pipe for sorting and speed reduction; the third chip unit C adopts a symmetrical rectangular serpentine Pipeline for further sorting and speed reduction; the fourth chip unit D adopts a serpentine pipe with a symmetrical rectangular section for final sorting and speed reduction; the fifth chip unit E is used for outlet mat...
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The invention discloses a 3D (three-dimensional) stacked multi-stage inertial microfluidic sorting chip and a preparation method of the 3D stacked multi-stage inertial microfluidic sorting chip. The chip comprises six layers of chip units which are stacked in sequence, the first chip unit is provided with a spiral pipeline with a trapezoidal cross section. And the second chip unit is provided with a second-stage main snakelike pipeline. And the third chip unit is provided with a third-stage main snakelike pipeline. And the fourth chip unit is provided with a fourth-stage main snakelike pipeline. According to the invention, after each layer of structure is prepared by a planar process, the preparation is simple by virtue of the mark points and mutual stacking and bonding, and the integration level of the multi-stage micro-fluidic chip is improved. According to the prepared chip, the solution of the mixed sample only needs to be input at the specific flow speed, automation of efficient sorting and speed reduction can be achieved, and the obtained sample solution can be directly connected with an imaging detection system for next-step analysis and diagnosis. In addition, the three modes can be switched by blocking part of the outlets so as to meet different sorting requirements.
Description
technical field [0001] The invention relates to cell sorting technology and microfluidic technology, in particular to microfluidic sorting technology based on the principle of inertia. Background technique [0002] Disease diagnosis and drug analysis often require purified target samples, such as cancer or sepsis, which need to be separated from blood to obtain circulating tumor cells or white blood cells. However, the amount of target cells used for disease diagnosis is very small in blood, so how to separate the target sample from the heterogeneous background of a large number of cells is extremely important. This is why cell sorting technology, as a key technology in the preparation of biological samples, has been receiving much attention. Nowadays, cell sorting techniques for clinical application have matured, including density gradient centrifugation, fluorescence-activated cell sorting, and magnetic-activated cell sorting. However, these technologies rely on professi...
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Login to View More IPC IPC(8): B01L3/00C12M1/00C12M1/34
Inventor 黄汐威徐雪峰
Owner HANGZHOU DIANZI UNIV