Composition for inhibiting generation of nitrosamine by combining plant essential oil with strain volatile substances and preparation method of composition
A technology of volatile substances and plant essential oils, which is applied in the field of composition and preparation of plant essential oils combined with volatile substances of bacterial strains to inhibit the formation of nitrosamines. Production of nitrosamines and the effect of reducing the production of nitrosamines
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] The preparation of embodiment 1 bacterial strain freeze-dried powder
[0033] 1. Activation of Bacillus Provence
[0034] (1) Liquid medium activation: use LB liquid medium, take 1.25g and dissolve it in 50ml water, sterilize at 121°C for 15 minutes, take 1ml of the bacterial liquid and inoculate it into the cooled liquid medium, at 37°C, 140r / min, cultivated for 36 hours.
[0035] (2) Separation and purification of solid medium: use LB nutrient agar medium, take 2g in 50ml water, sterilize at 121°C for 15 minutes, pour the plate after the medium is cooled to 50°C, after the plate is cooled, take 20μl of bacterial suspension in Solid medium, cultured at 37°C for 36 hours after streaking, and picked a single colony for culture.
[0036] (3) Liquid medium culture: use LB liquid medium, take 1.25g and dissolve it in 50ml water, sterilize at 121°C for 15 minutes, take 1ml of the bacterial liquid and inoculate it into the cooled liquid medium, at 37°C, 140r / m min, cultiv...
Embodiment 2
[0045] Embodiment 2 product preparation
[0046] Mix 1 ml each of grapefruit essential oil and 1 ml of cinnamon essential oil by volume with 1 g each of the freeze-dried powder of the 48-h liquid culture of Bacillus provence and Stenotrophomonas rhizophilus prepared in Example 1, and mix evenly.
Embodiment 3
[0047] Embodiment 3 product preparation
[0048] Mix 1ml each of grapefruit essential oil and cinnamon essential oil by volume with 1g each of the lyophilized powder of the 48h liquid culture of Bacillus provence and Stenotrophomonas rhizophilus prepared in Example 1, and the product uses a breathable packaging material Package.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com