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Preparation method of exopolysaccharide and intracellular polysaccharide of Fraxinus chinensis and application of exopolysaccharide and intracellular polysaccharide in regulating intestinal microbial flora and reducing blood sugar

A technology of intracellular polysaccharides and white wax, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems that have not been discovered, and achieve the effect of reducing relative abundance, increasing relative abundance, and high content

Active Publication Date: 2022-04-08
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no research has been found on the effect of the polysaccharides of the perennial polysaccharides on the intestinal microbiota to exert the hypoglycemic effect

Method used

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  • Preparation method of exopolysaccharide and intracellular polysaccharide of Fraxinus chinensis and application of exopolysaccharide and intracellular polysaccharide in regulating intestinal microbial flora and reducing blood sugar
  • Preparation method of exopolysaccharide and intracellular polysaccharide of Fraxinus chinensis and application of exopolysaccharide and intracellular polysaccharide in regulating intestinal microbial flora and reducing blood sugar
  • Preparation method of exopolysaccharide and intracellular polysaccharide of Fraxinus chinensis and application of exopolysaccharide and intracellular polysaccharide in regulating intestinal microbial flora and reducing blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Embodiment 1: Preparation of intracellular polysaccharide of perennial bacteria

[0090] (1) Perennial perennial ash is activated by bacterial classification, cultured on a slanted surface and submerged in liquid fermentation to obtain mycelium of perennial perennial ash. The specific methods are as follows:

[0091] ①Cultivation of slant strains: After activation and cultivation of the slant parent species of Perennial aureus, inoculate in sterilized slant solid medium, and cultivate at 28°C for 8 days for later use;

[0092] ② Liquid submerged fermentation culture: pick 4 to 6 soybean-sized bacteria blocks from the slant of the slanted strain of the white wax perennial bacteria, inoculate in 150mL sterile liquid fermentation medium, and shake at 30°C and 180rpm at a constant temperature in the dark Cultivate for 9 days to obtain the liquid fermentation product of the perennial bacteria, and separate the mycelium by centrifugation and vacuum filtration, and dry it at 6...

Embodiment 2

[0102] Embodiment 2: Preparation of intracellular polysaccharide of perennial bacteria

[0103] (1) Perennial perennial ash is activated by bacterial classification, cultured on a slanted surface and submerged in liquid fermentation to obtain mycelium of perennial perennial ash. The specific methods are as follows:

[0104] ①Cultivation of slant strains: After activation and cultivation of the slant parent species of Perennial spp., inoculate in sterilized slant solid medium, culture at 28°C for 8 days, and set aside;

[0105] ② Liquid submerged fermentation culture: pick 4 to 6 soybean-sized bacteria blocks from the slant of the slanted strain of the white wax perennial bacteria, inoculate in 150mL sterile liquid fermentation medium, and shake at 30°C and 180rpm at a constant temperature in the dark Cultivate for 9 days to obtain the liquid fermentation product of Perennial aceriformis, which is separated by centrifugation and vacuum filtration, and dried at 60° C. to obtain ...

Embodiment 3

[0115] Embodiment 3: Preparation of intracellular polysaccharide of perennial bacteria

[0116] (1) Perennial perennial ash is activated by bacterial classification, cultured on a slanted surface and submerged in liquid fermentation to obtain mycelium of perennial perennial ash. The specific methods are as follows:

[0117] ①Cultivation of slant strains: After activation and cultivation of the slant parent species of Perennial spp., inoculate in sterilized slant solid medium, culture at 28°C for 8 days, and set aside;

[0118] ② Liquid submerged fermentation culture: pick 4 to 6 soybean-sized bacteria blocks from the slant of the slanted strain of the white wax perennial bacteria, inoculate in 150mL sterile liquid fermentation medium, and shake at 30°C and 180rpm at a constant temperature in the dark Cultivate for 9 days to obtain the liquid fermentation product of Perennial aceriformis, which is separated by centrifugation and vacuum filtration, and dried at 60° C. to obtain ...

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Abstract

The invention belongs to the technical field of biology, and particularly discloses a preparation method of exopolysaccharide and intracellular polysaccharide of Fraxinus chinensis and application of the exopolysaccharide and the intracellular polysaccharide in regulating intestinal microbial flora and reducing blood sugar. The exopolysaccharide and the intracellular polysaccharide of the Fraxinus chinensis can be used as prebiotics, not only can regulate intestinal bacterial flora structures of diabetic mice, improve relative abundance of intestinal flora such as lactobacillus, spirillum, faecalis bacillus and bacteroides and reduce relative abundance of bacillus, campylobacter and enterobacter, but also can regulate fungal flora structures, so that the intestinal bacterial flora structure of the diabetic mice is improved, and the intestinal bacterial flora structure of the diabetic mice is improved. The number ratio of basidiomycota to ascomycomycota is reduced, the relative abundance of pro-inflammatory fungi such as aspergillus and mortierella is reduced, and the relative abundance of penicillium and candida albicans is improved. In addition, both the exopolysaccharide and the intracellular polysaccharide of the Fraxinus chinensis can improve the insulin level, the glycosylated hemoglobin level and the functions of pancreas islet beta cells of the mouse so as to achieve the effect of improving the blood sugar of the diabetic mouse.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for preparing exopolysaccharides and intracellular polysaccharides of perennial bacteria and its application in regulating intestinal microbial flora and lowering blood sugar. Background technique [0002] Perenniporia fraxinea belongs to Basidiomycotina, Agaricomycetes, Polyporales, Polyporaceae, and Perenniporia. Medicinal fungi. White wax perennial bacteria contain a variety of physiologically active components, including polysaccharides, polyphenols, plasminase and so on. Studies have shown that perennial perennials has anti-tumor effect and antioxidant activity, but there are relatively few studies on the extraction method and medicinal value of polysaccharides from perennial perennials perennials. [0003] Bacteria, archaea, and colonic fungi in the gut are collectively referred to as "gut microbes." The number of microorganisms in the gastrointe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04A61K31/715A61P1/00A61P3/10C12R1/645
Inventor 张松郑晓雅
Owner SOUTH CHINA NORMAL UNIVERSITY
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