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Gene group for breast cancer molecular typing, detection product and application thereof

A molecular typing and breast cancer technology, applied in the biological field, can solve problems such as physical injury of patients, and achieve the effect of alleviating pain and avoiding injury.

Pending Publication Date: 2022-04-08
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem in the prior art that the molecular typing of breast cancer requires tissue resection and harm to the patient's body, the present invention provides a set of gene groups that can be used for molecular typing of breast cancer

Method used

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  • Gene group for breast cancer molecular typing, detection product and application thereof
  • Gene group for breast cancer molecular typing, detection product and application thereof
  • Gene group for breast cancer molecular typing, detection product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 There are significant differences in sEVs proteins in patients with four subtypes of breast cancer

[0031] The present invention takes plasma from 20 patients with lumen A breast cancer, 50 patients with lumen B type, 21 patients with HER2 enrichment type and 23 patients with triple negative breast cancer, and extracts the sEVs protein in the plasma. The extracted sEVs proteins were analyzed by UHPLC-QExactive HF MS to separate and identify which sEVs proteins were expressed in each of the above four subtypes, and to obtain the expression abundance of each protein. Furthermore, in order to detect whether there are differences in the sEVs proteins in the four subtypes of people, so that these sEVs proteins can be used for molecular typing, the present invention further performs PCA analysis on these sEVs proteins.

[0032] 1. Experimental method

[0033] 1. sEVs protein extraction

[0034] (1) Differential ultracentrifugation purification of sEVs: First, 1 m...

Embodiment 2

[0045] Example 2 Obtaining significantly differentially expressed sEVs proteins in patients of each subtype

[0046] Further, the present invention obtains significantly differentially expressed sEVs proteins in patients of each subtype through t-test and nominal p-value cutoff (p<0.05) analysis.

[0047] 1. Experimental method

[0048] t-test and nominal p-value cut-off (p<0.05) analysis: 1924 specifically expressed sEVs proteins were analyzed by t-test, and the sEVs proteins that were significantly differentially expressed in breast cancer patients were determined by calculating the fold difference. The determination method is:

[0049] Fold difference = the amount of the protein expressed in one of the subtypes of breast cancer patients / the amount of the protein expressed in the other subtypes of patients

[0050] If the multiples of difference are all greater than 2, it is considered that the protein is significantly differentially expressed in the breast cancer subtype ...

Embodiment 3

[0053] Example 3 Construction of breast cancer subtype typing model

[0054] Based on the above-mentioned 356 significantly differentially expressed sEVs proteins, the present invention adopts the XGBoost classification method to construct a subtype model of breast cancer, in order to obtain a model capable of molecular typing of breast cancer. It prevents tissue biopsy from harming the patient's body and reduces the patient's pain in seeing a doctor.

[0055] 1. Experimental method

[0056] The XGBoost classification method is an existing classification method that is robust to noise and overfitting. Based on the XGBoost classification method, the present invention imports the above-mentioned 356 significantly differentially expressed sEVs protein data into the XGBoost classification model, uses 70% of the samples of the above breast cancer subtypes as a training set, and the remaining 30% as an independent test set to carry out the model train.

[0057] 2. Experimental re...

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Abstract

The invention discloses a gene group for breast cancer molecular typing, a detection product and application thereof, and the gene group for breast cancer molecular typing comprises at least one of PSMB7, PSMB2, FAP, CAPN2, YWHAB, YWHAG and YWHAH, at least one of RAB10, PKLR, GOT1, STAT1, ALAD, VCAM1 and HSPD1, at least one of PRDX1, TXN, SOD3, FABP5, GAA, BPGM, GAPDH, CASP14, KRT17 and TGM3, and AHSG, ACTN4, PPBP, TLN1, PF4, EHD1, COL1A1, PROC, COL1A2, F11, PRKACB and DBNL.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to gene groups, systems and applications for molecular typing of breast cancer. Background technique [0002] Breast cancer (BC) is one of the most common cancers worldwide, accounting for 30% of cancers in women (Kim, J.S., Chang, J.W., Park, J.K., and Hwang, S.G. (2012). Increased aldehyde reductase expression mediated acquired radioresistance of laryngeal cancer cells via modulating p53. Cancer Biol Ther 13, 638-646.). Since the mid-1970s, there has been a long-term decline in mortality due to improvements in treatment options, including chemotherapy, immunotherapy, and the development of targeted therapies (Cassetta, L., and Pollard, J.W. (2017). Repolarizing macrophages improves breast cancer therapy. Cell Res 27, 963-964.). [0003] Invasive breast cancer (IBC) is a highly heterogeneous disease that can be divided into various intrinsic or molecular subtypes that are associated ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86G16B40/00G06K9/62
Inventor 王红霞杜少倩
Owner SHANGHAI FIRST PEOPLES HOSPITAL