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Application of reagent for detecting TRIM45 expression level in preparation of hepatocellular carcinoma diagnosis and treatment preparation

A technology for hepatocellular carcinoma and expression level, which can be used in biological tests, measurement devices, material inspection products, etc., and can solve the problem of increasing the difficulty of studying the molecular mechanism of HCC

Pending Publication Date: 2022-04-12
JIANGSU VOCATIONAL COLLEGE OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The complexity of etiology increases the difficulty of studying the molecular mechanism of hepatocellular carcinoma

Method used

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  • Application of reagent for detecting TRIM45 expression level in preparation of hepatocellular carcinoma diagnosis and treatment preparation
  • Application of reagent for detecting TRIM45 expression level in preparation of hepatocellular carcinoma diagnosis and treatment preparation
  • Application of reagent for detecting TRIM45 expression level in preparation of hepatocellular carcinoma diagnosis and treatment preparation

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Experimental program
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Embodiment 1

[0016] Data download and statistical analysis

[0017] From https: / / portal.gdc.cancer.gov / website project select "TCGA-LIHC", experimental.strategy select "RNA-Seq", data.category select "TranscriptomeProfiling", data.type select "Gene ExpressionQuantification", WorkflowType Select "HTSeq-FPKM". Clinical information (LIHC_clinicalMatrix) and survival information (LIHC_survival) were downloaded from the http: / / xena.ucsc.edu / website. The samples with complete survival data were filtered out, and the samples included 369 hepatocellular carcinoma (HCC) tissues and 50 normal tissues. Among them, 339 patients had complete gene expression and clinical information data. The expression data was transformed by log2, and the tumor tissue and normal tissue were subjected to t-test, and the threshold was set to |log2(foldchange)|>1 and Adjustedpvalue<0.05 to obtain differentially expressed genes. The result was that TRIM45 was upregulated in HCC tissues. The ROC curve was drawn and t...

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Abstract

The invention belongs to the technical field of biology, and relates to application of a reagent for detecting TRIM45 expression level in preparation of a hepatocellular carcinoma diagnosis and treatment preparation. The invention finds that TRIM45 is related to occurrence and development of hepatocellular carcinoma for the first time. Through bioinformatics analysis, it is found that TRIM45 has a high AUC value in hepatocellular carcinoma patients, and it is prompted that TRIM45 serving as the diagnosis marker of hepatocellular carcinoma has high accuracy and specificity. According to mRNA-seq data and clinical data from a cancer genome map database, it is verified that the expression level of TRIM45 can affect survival of hepatocellular carcinoma patients, it is prompted that TRIM45 has correlation with hepatocellular carcinoma, research on pathogenesis of hepatocellular carcinoma will be further enriched, and the application of the TRIM45 to the hepatocellular carcinoma is promoted. And a novel tumor marker and a novel therapeutic target are provided for diagnosis and prognosis monitoring of hepatocellular carcinoma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a reagent for detecting the expression level of TRIM45 in the preparation of preparations for the diagnosis and treatment of hepatocellular carcinoma. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignancies of the digestive system with increasing mortality worldwide. Many risk factors, including viral infection, fibrosis, alcohol use, and metabolic deregulation, induce genetic or phenotypic changes and contribute to tumor progression. The complexity of the etiology increases the difficulty of studying the molecular mechanism of HCC. Early-stage treatments for HCC such as liver transplantation, tumor resection, or ablation. Due to the poor prognosis of hepatocellular carcinoma, the 5-year survival rate of patients is particularly low, so early detection of tumors and more personalized treatment are new goals ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574G01N33/68
Inventor 郭元
Owner JIANGSU VOCATIONAL COLLEGE OF MEDICINE