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Cell chip as well as detection method and application of cell chip for bacterial infection

A technology of cell chip and preparation method, which is applied in the direction of measurement device, analysis by causing chemical reaction of materials, and material analysis by observing the influence on chemical indicators, etc., which can solve the incomparability of results of detection antibodies and data presentation methods and other issues, to achieve the effect of reducing economic burden, reducing the abuse of antibiotics, and simple operation

Pending Publication Date: 2022-04-12
何骏杰
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  • Abstract
  • Description
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Problems solved by technology

The expression of CD64 is usually detected by whole blood surface staining and multi-color flow cytometry (FlowCytoMetry, FCM), but the inconsistency of flow cytometer models, detection antibodies and data presentation methods in different hospitals may cause incomparability of results

Method used

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  • Cell chip as well as detection method and application of cell chip for bacterial infection
  • Cell chip as well as detection method and application of cell chip for bacterial infection
  • Cell chip as well as detection method and application of cell chip for bacterial infection

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Embodiment Construction

[0036]Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application. Various commonly used reagents used in the examples are all commercially available products.

[0037] The printing ink described in the embodiment of the present application can adopt Shanghai Deli Stationery Co., Ltd., the article number is: S558; The described aldehyde-modified glass slide can adopt German Schott (SCHOTT) company, and the article number is 1064874; The anti-mouse polyclonal secondary antibody can be used from B...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a cell chip and a detection method and application thereof for bacterial infection.The cell chip comprises an aldehyde group modified glass slide, the surface of the glass slide is divided into at least one reaction area, a polyclonal secondary antibody is fixed in the reaction area, the polyclonal secondary antibody is connected with a CD64 monoclonal primary antibody, and the CD64 monoclonal primary antibody is connected with a CD64 monoclonal secondary antibody. The preparation method of the cell chip for bacterial infection detection comprises the following steps: dropwise adding a mixed solution of a polyclonal secondary antibody and a CD64 monoclonal primary antibody prepared according to a mass ratio of 1: (0.2-4) into a reaction area on the surface of a positioning glass slide, incubating for 30-60 minutes, then leaching and soaking with a buffer solution, and finally drying to obtain the cell chip. The invention aims to provide a cell chip which is low in cost and simple and convenient to operate and can be used for absolute counting of CD64 neutrophils. A CD64 biological marker which is high in specificity, high in sensitivity and capable of being continuously detected is adopted in the cell chip.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cell chip and a detection method and application thereof for bacterial infection. Background technique [0002] Infectious disease is one of the most common clinical diseases, and it is also one of the main causes of death in intensive care unit (Intensive Care Unit, ICU) patients. Currently, laboratory methods for clinical diagnosis of bacterial infection mainly include bacterial culture, blood routine, C-reactive protein (CRP), procalcitonin (PCT), etc. [0003] However, bacterial culture is the gold standard for judging infection, but it takes a long time, ranging from 3 to 7 days at least, and longer at more. The results are easily affected by many external factors, such as specimen collection time, method, delivery method, and the use of antibacterial drugs, etc., which may easily cause false negatives and / or false positives; blood routine as an inflammatory indic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N21/78G01N21/84
Inventor 何骏杰李文娜张舒汤莹
Owner 何骏杰
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