A strain of Lactobacillus plantarum lp1z that produces lysozyme and efficiently antagonizes multidrug-resistant Helicobacter pylori and its application

A technology of Lactobacillus plantarum and Helicobacter pylori is applied in the field of microorganisms to achieve the effects of inhibiting growth, relieving inflammation and high survival rate

Active Publication Date: 2022-05-27
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is currently no research that clearly indicates that probiotics can solve the current problem of multidrug-resistant Hp infection

Method used

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  • A strain of Lactobacillus plantarum lp1z that produces lysozyme and efficiently antagonizes multidrug-resistant Helicobacter pylori and its application
  • A strain of Lactobacillus plantarum lp1z that produces lysozyme and efficiently antagonizes multidrug-resistant Helicobacter pylori and its application
  • A strain of Lactobacillus plantarum lp1z that produces lysozyme and efficiently antagonizes multidrug-resistant Helicobacter pylori and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Isolation and identification of Lactobacillus plantarum LP1Z

[0047] 1.1. Isolation and preservation of Lactobacillus plantarum LP1Z

[0048] The feces of healthy centenarians in Jiaoling County, Meizhou City, Guangdong Province, China, the hometown of longevity in the world, were collected as samples. In a sterile environment, 0.1 g of feces was added to 10 ml of MRS liquid culture medium, and the mixture was shaken and mixed at 37°C. The enrichment culture was carried out under oxygen conditions for 24 hours, and 0.5 ml of bacterial liquid was drawn for serial dilution. Add normal saline to make 10 -1 to 10 -8 Dilute the gradient bacterial suspension, choose 10 -6 , 10 -7 , 10 -8 Pipette 100 μl of the three gradient bacterial suspensions into MRS agar medium, spread them evenly with a spreader, and then culture them under anaerobic conditions at 37°C for 48h. Pick a typical colony on the plate to MRS agar medium for streak purification, pick a single ...

Embodiment 2

[0051] Example 2 Cultivation of Lactobacillus and Preparation of Lactobacillus Fermentation Liquid

[0052] 2.1. Culture of Lactobacillus

[0053] Lactobacillus plantarum LP1Z was inoculated onto MRS agar plates from a glycerol tube, and cultured anaerobically at 37°C for 48h, and the colony morphology was observed. The colony on the surface of Lactobacillus plantarum is about 3mm in diameter, convex, round, and the surface is smooth, dense, white, and occasionally light yellow. The bacteria were observed under a microscope, and it was found that Lactobacillus plantarum was Gram-positive, and the bacteria were short rods with round ends.

[0054] A single colony of Lactobacillus plantarum LP1Z was picked and inserted into the MRS broth medium and cultured anaerobically at 37°C for 48h to make a growth curve. It was found that the bacteria was cultured at 37°C for 12h to reach a stable phase, and its fermentation method was heterozygous fermentation, which could produce acid ...

Embodiment 3

[0057] Example 3 Evaluation of the in vitro anti-Helicobacter pylori ability of Lactobacillus plantarum LP1Z

[0058] 3.1. Culture of Helicobacter pylori

[0059] Helicobacter pylori ATCC 43504 and GDMCC No: 61571 were purchased from the Guangdong Provincial Collection of Microorganisms, of which ATCC 43504 was isolated from a chronic gastritis patient in Australia; GDMCC No: 61571 (published in patent application CN202110612046.9) was isolated from my country Gastric mucosa tissue samples from a patient with chronic gastritis in Guangdong were resistant to five antibiotics, amoxicillin, metronidazole, clarithromycin, levofloxacin, and tetracycline.

[0060] Activated from the seed preservation tube, pipet 100 μL of seed preservation solution and spread it on a Helicobacter pylori selective medium plate containing 10% sterile fetal bovine serum. 2 , 10%CO 2 , 85%N 2 ) for 5-7 days. Pick the typical colonies on the plate to the Helicobacter pylori selective culture plate for...

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Abstract

The invention discloses a strain of Lactobacillus plantarum LP1Z which produces lysozyme and efficiently antagonizes multidrug-resistant Helicobacter pylori and its application. Lactobacillus plantarum LP1Z, which was preserved in Guangdong Microbial Culture Collection Center on February 21, 2022, address: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, Guangdong Province, postcode: 510070, and the preservation number is GDMCC No :62256. (1) The probiotics involved in the present invention can produce various active antibacterial substances such as organic acid and lysozyme, and can effectively inhibit the growth of Helicobacter pylori in the stomach; Colonization, the body can compete with pathogenic bacteria for binding sites, effectively reducing the infection rate of Helicobacter pylori in the stomach; (3) It can improve the pathological state of the stomach and relieve inflammation. Therefore, Lactobacillus plantarum LP1Z has great application prospects in the preparation of products (such as medicines) for preventing or treating Hp infection, especially multidrug-resistant Hp infection.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and the technical field of medicine, in particular to a Lactobacillus plantarum LP1Z which produces lysozyme and efficiently antagonizes multidrug-resistant Helicobacter pylori and its application. Background technique [0002] Helicobacter pylori ( Helicobacter pylori , Hp) is a Gram-negative, microaerophilic helical bacterium, its infection can cause a variety of gastrointestinal diseases and is one of the key factors leading to gastritis, gastric ulcer and even gastric cancer. In 1994, Hp was identified as a class I carcinogen by the World Health Organization, so the research on the prevention and treatment of Helicobacter pylori has great application value. [0003] Global scholars pointed out in the Maastricht / Florence consensus released in 2011 that all patients with confirmed H. pylori infection require eradication therapy. The guidelines also pointed out that the main treatment fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/689C12N15/11C12N9/24A61K35/747A61P31/04A61P1/00A23L33/135C12R1/25
CPCY02A50/30
Inventor 吴清平商燕燕李滢谢新强丁郁王涓薛亮陈谋通张菊梅叶青华吴诗陈惠元吴军林
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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