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Human tumor xenotransplantation model construction method and application thereof

A technology of xenotransplantation and construction method, which is applied in animal husbandry and other fields, can solve the problems of long tumor formation time, less blood volume in mice, and low implantation success rate, and achieve the effect of increasing tumor formation rate and shortening tumor formation time

Pending Publication Date: 2022-04-22
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the establishment of human tumor xenograft models in different types of immunodeficient mice has problems such as low implantation success rate, long tumor formation time, less blood volume in mice, and insufficient tumor tissue, which seriously hinders the basic research on tumors and drug development. Evaluation

Method used

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  • Human tumor xenotransplantation model construction method and application thereof
  • Human tumor xenotransplantation model construction method and application thereof
  • Human tumor xenotransplantation model construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Breeding of IL2RG knockout Syrian hamsters

[0036] Select female heterozygous IL2RG knockout Syrian hamsters over 6 weeks old and male hemizygous IL2RG knockout Syrian hamsters over 8 weeks old. The female mice were kept in single cages, and the production was observed from the 16th day to the 28th day ( figure 1 ). From hamsters 12 days after birth, the genome (gDNA) was extracted and the genes were identified. Using the extracted hamster gDNA as a template, the upstream and downstream primers were designed at the position of the gene mutation, namely IL2RGT_F (5'-CCTGAACTTAGGTGACCTGA-3') and IL2RGT_R (5-CTAAGTTTGAGAACCAAATG-3'), PCR amplification reaction was performed, PCR Amplification yielded a fragment of about 606 bp in length. The obtained PCR amplification products were subjected to Sanger sequencing to identify whether the Syrian hamsters were homozygous, heterozygous and wild-type, and the DNA PCR amplification products of wild-type Syrian hamst...

Embodiment 2

[0038] Example 2 Construction of human tumor xenograft model using IL2RG gene knockout Syrian hamster

[0039] The present embodiment utilizes the IL2RG gene knockout Syrian hamster to construct a human-derived tumor xenograft model, and the specific method is as follows:

[0040] 1. The tumor tissue blocks of the patient were excised under sterile conditions, which were respectively lung cancer tumor tissue block, esophageal cancer tumor tissue block, and lymphoma tissue block. The tumor tissue block and the lymphoma tissue block were washed three times, and the lung cancer tumor tissue block, esophageal cancer tumor tissue block, and lymphoma tissue block were divided into 4 mm each. 3 The inoculated tumor mass was obtained to obtain lung cancer tumor tissue mass, esophageal cancer tumor tissue mass, and lymphoma tissue mass.

[0041]2. In the modeling group, 5-6 weeks old, male or female (hamster weight is 100g), IL2RG gene knockout Syrian hamsters are used for subcutaneou...

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Abstract

The invention relates to a human tumor xenotransplantation model construction method and application thereof. According to the construction method of the humanized tumor xenotransplantation model of the narrative hamster based on the IL2RG gene knockout immunodeficiency, provided by the invention, the personalized humanized narrative hamster tumor xenotransplantation model is successfully established by transplanting tumor cells or tissues of a patient. The method is high in tumor formation rate and short in tumor formation time, and more human-derived tumor samples can be obtained. The constructed human-derived tumor xenotransplantation model retains matrix heterogeneity, histological characteristics, molecular diversity and microenvironment of primary tumors, can be widely applied to the aspects of preclinical drug effect personalized screening evaluation, biomarker identification and the like, and lays a foundation for tumor fundamental research and drug evaluation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for constructing a human-derived tumor xenograft model and its application. Background technique [0002] Malignant tumors are one of the diseases that threaten human health and safety. The morbidity and mortality of tumors are high, and the efficiency of effective clinical treatment is still low. With the deepening of tumor molecular biology and genomics research, human beings have a more comprehensive understanding of the characteristics of tumor heterogeneity. Genome sequencing studies have found that there are few gene mutation duplications between tumors of the same pathological type; the gene mutation spectrum between different individuals of the same type of tumor is very different, which is the biological basis for the different clinical phenotypes of the same pathological type of tumor It is also the reason why different individuals of the same ...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 苗晋鑫
Owner HENAN UNIV OF CHINESE MEDICINE
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