Large-scale breeding method for all-male freshwater shrimps

Abstract

The invention discloses a method for large-scale breeding of all-male freshwater shrimps. The method comprises the following steps: step 1, preparing dsRNA in vitro according to a sequence of a freshwater shrimp gene sequence; 2, transfecting the obtained dsRNA prawn seeds, and detecting the interference effect; 3, the freshwater shrimps subjected to RNA interference are captured when the body length is 3 + / -0.5 cm, and the sex condition of the male and female shrimps is identified; carrying out molecular biological sex identification on the shrimps with the phenotypes of female shrimps; step 4, cultivating and verifying all-male freshwater shrimps; 5, passage of all-male freshwater shrimp female parent false female shrimps; the problem that the breeding density is too high due to freshwater shrimp breeding can be effectively solved, male shrimps grow fast, molt synchronism is high, the killing rate is low, breeding management is convenient, and the breeding yield is increased.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for large-scale breeding of all male freshwater shrimp. Background technique [0002] Freshwater prawn, also known as river prawn, scientific name giant river prawn, is an important freshwater prawn species in my country because of its delicious meat and high nutritional value. There is a significant gender difference between male and female freshwater shrimp. The male shrimp is larger and grows faster, which has a growth advantage. In the market, large-sized freshwater shrimp have obvious price advantages. [0003] During the freshwater shrimp breeding process, the female shrimps begin to mature sexually after more than 2 months after the seedlings are released, periodically laying eggs and hatching to produce a large number of larvae. The reproduction of female shrimp leads to high density, which affects the growth rate, and the final market size is small, which aff...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the deficiencies in the prior art, the object of the present invention is to provide a method for large-scale breeding of all male freshwater shrimps, which can solve the problem of high breeding density caused by self-propagation in the freshwater shrimp cultivation process, slow growth rate, and large-scale shrimp ratio. The low problem; the shrimp seedlings bred by this method have a high male ratio, fast growth and good economic benefits

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