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Extraction, purification and filtration integrated purification column and application thereof

A purification column and integrated technology, applied in the direction of filtration separation, filtration circuit, chemical instruments and methods, etc., can solve the problems of cumbersome operation steps, low efficiency, cumbersome purification and extraction process, etc., and achieve good purification effect

Pending Publication Date: 2022-04-22
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunoaffinity columns and multifunctional purification columns have cumbersome, complicated, and time-consuming operating steps
[0003] In summary, in the existing methods for extracting and purifying mycotoxins, pesticide residues, and veterinary drug residues, the purification and extraction process is cumbersome, low in efficiency, and poor in effect. It is urgent to develop a new integrated purification column that integrates extraction, purification, and filtration. and detection method

Method used

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  • Extraction, purification and filtration integrated purification column and application thereof
  • Extraction, purification and filtration integrated purification column and application thereof
  • Extraction, purification and filtration integrated purification column and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Such as figure 1 As shown, the present invention provides an integrated purification column integrating extraction, purification and filtration. The purification column includes a hollow column body 100. Specifically, the cavity column body 100 is divided into an extraction column body and a purification column body. The purification column body The diameter is smaller than the diameter of the extraction cylinder. A first sieve plate 110, a second sieve plate 120, and a third sieve plate 130 are arranged in sequence along the liquid flow direction in the cavity cylinder 100, and a purification filler is filled between the second sieve plate 120 and the third sieve plate 130, Among them, the purification packing of trichothecene integrated purification column is a mixture of 200mg GCB, 100mg C18-CX, 100mg MCX and 50mg POLY;

[0031] The purification packing of the multi-mycotoxin integrated purification column is a mixture of 200mg C18-CX, 100mg MCX and 100mg POLY.

[...

Embodiment 2

[0050] Such as figure 1 As shown, this embodiment provides an integrated purification column integrating extraction, purification, and filtration. The integrated purification column includes a cavity column 100. Specifically, the cavity column 100 is divided into an extraction column and a purification column. The cleanup cartridge has a smaller diameter than the extraction cartridge. A first sieve plate 110 , a second sieve plate 120 and a third sieve plate 130 are arranged in sequence along the flow direction of the liquid in the cavity cylinder 100 , and a cleaning filler is filled between the second sieve plate 120 and the third sieve plate 130 . The second sieve plate 120 and the third sieve plate 130 are arranged in the purification column, and the purification filler is a mixture of 50mg GCB, 200mg C18-CX, 100mg MCX and 100mg POLY.

[0051] In the purification cylinder of the present invention, the cavity cylinder 100 is further provided with a filter membrane, and the...

Embodiment 3

[0064] Such as figure 1 As shown, the present invention provides an integrated purification column integrating extraction, purification and filtration. The purification column includes a hollow column body 100, which is divided into an extraction column body and a purification column body. The diameter of the purification column body is smaller than the diameter of the extraction cartridge. A first sieve plate 110 , a second sieve plate 120 and a third sieve plate 130 are arranged in sequence along the flow direction of the liquid in the cavity cylinder 100 , and a cleaning filler is filled between the second sieve plate 120 and the third sieve plate 130 . The second sieve plate 120 and the third sieve plate 130 are arranged in the purification column, and the purification filler is a mixture of 200mg C18-CX and 200mg POLY.

[0065] In the purification cylinder of the present invention, the cavity cylinder 100 is further provided with a filter membrane, and the filter membran...

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Abstract

The invention discloses an integrated purification column integrating extraction, purification and filtration and application thereof.The integrated purification column comprises a cavity column body, a first sieve plate, a second sieve plate and a third sieve plate are sequentially arranged in the cavity column body in the fluid flowing direction, and the space between the second sieve plate and the third sieve plate is filled with purification filler; and a filter membrane is arranged below the third sieve plate. According to the integrated purification column disclosed by the embodiment of the invention, a sample does not need to be centrifuged, filtered and repeatedly transferred after being extracted, the sample directly enters the integrated purification column to be purified through the sieve plate under the action of gravity, and the sample can be directly detected after being filtered, so that the pretreatment of the sample is controlled within 30 minutes from 60 minutes; and the cost increase and environmental pollution caused by the use of consumables such as centrifugal tubes, gun heads, injectors and the like are reduced.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an integrated purification column integrating extraction, purification and filtration and its application. Background technique [0002] In order to monitor mycotoxin contamination, pesticide residues, and veterinary drug residues in grain, oil, feed, and food, it is urgent to develop simple and rapid methods for detecting mycotoxins, pesticides, and veterinary drugs in grain, oil, feed, and food. The existing purification methods are mainly immunoaffinity column purification and multifunctional purification column purification. Immunoaffinity columns and multifunctional purification columns have the problems of cumbersome, complicated and time-consuming operation steps. [0003] In summary, in the existing methods for extracting and purifying mycotoxins, pesticide residues, and veterinary drug residues, the purification and extraction process is cumbersome, low in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N30/06B01D36/00
CPCG01N1/34G01N30/06B01D36/00
Inventor 叶金吴宇王松雪李丽
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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