In-vitro tissue culture propagation method for snail begonia

A technology for begonias and snails, applied in the field of in vitro tissue culture reproduction of snail begonias, can solve the problems of low reproduction coefficient, unable to meet market demand, difficult to scale production, etc. Effect

Pending Publication Date: 2022-04-29
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of low cutting propagation coefficient of snail begonia, difficulty in forming large-scale production in a short period of time, and inability to meet market demand, the present invention provides a method for in vitro tissue culture propagation of snail begonia, through which the propagation coefficient is increased and the high-efficiency of snail begonia is promoted. Breeding, forming large-scale production as soon as possible to meet market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The present invention is accomplished through the following technical solutions: A method for in vitro tissue culture propagation of snails and begonias, which is characterized in that it comprises the following steps:

[0022] 1) Collect snail begonia leaves and place them in a refrigerator at 4°C for 15 h;

[0023] 2) After taking out the leaf in step 1), wipe it with alcohol with a mass concentration of 75%, and then disinfect it with a sodium hypochlorite solution with a mass concentration of 0.15% for 12 minutes, and then rinse it with sterile water for 3 times, each time for 30s;

[0024] 3) Put the leaves treated in step 2) on sterile filter paper, and cut into three 5×5cm square leaves;

[0025] 4) Inoculate the square leaves cut in step 3) on the medium of MS+6-BA 1.0 mg / L+NAA 0.5 mg / L, under the indoor conditions of 70% relative air humidity and 12 hours of light, After cultivating for 7 days, the leaves were slightly raised, and after further culturing under...

Embodiment 2

[0030] The present invention is accomplished through the following technical solutions: A method for in vitro tissue culture propagation of snails and begonias, which is characterized in that it comprises the following steps:

[0031] 1) Collect the leaves of snail begonia and put them in the refrigerator at 4°C for 20 h;

[0032] 2) After taking out the leaf in step 1), wipe it with alcohol with a mass concentration of 75%, and then disinfect it with a sodium hypochlorite solution with a mass concentration of 0.15% for 20 minutes, and then rinse it with sterile water 4 times, 30s each time;

[0033] 3) Put the leaves treated in step 2) on sterile filter paper, and cut into four 5×5cm square leaves;

[0034] 4) Inoculate the square leaves cut in step 3) on the medium of MS+6-BA 1.0 mg / L+NAA 0.5 mg / L, under the indoor conditions of 80% relative air humidity and 10 hours of light, After culturing for 10 days, the leaves were slightly raised, and after further culturing under th...

Embodiment 3

[0039] The present invention is accomplished through the following technical solutions: A method for in vitro tissue culture propagation of snails and begonias, which is characterized in that it comprises the following steps:

[0040] 1) Collect the leaves of snail begonia and put them in the refrigerator at 4°C for 18 h;

[0041] 2) After taking out the leaves in step 1), wipe them with alcohol with a mass concentration of 75%, and then disinfect them with 0.15% sodium hypochlorite solution for 16 minutes, and then rinse them with sterile water 3 times, each time for 30s;

[0042] 3) Put the leaves treated in step 2) on sterile filter paper, and cut into three 5×5cm square leaves;

[0043] 4) Inoculate the square leaves cut in step 3) on the medium of MS+6-BA 1.0 mg / L+NAA 0.5 mg / L, under the indoor conditions of 75% relative air humidity and 11 hours of light, After culturing for 8 days, the leaves were slightly raised, and after further culturing under the same indoor condi...

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PUM

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Abstract

The invention provides a snail begonia in-vitro tissue culture propagation method. The snail begonia in-vitro tissue culture propagation method comprises the following steps: 1) collecting snail begonia leaves; (2) disinfecting the leaves; (3) cutting into square blades; (4) inoculating on a culture medium containing MS, 1.0 mg/L of 6-BA and 0.5 mg/L of NAA, and culturing under the indoor conditions that the relative air humidity is 70-80% and the illumination is 10-12 hours; (5) inoculating to a culture medium containing MS, 1.0 mg/L of 6-BA and 0.5 mg/L of NAA for multiplication culture; the method comprises the following steps of 1, inoculating a culture medium containing 1/2MS, 0.1 mg/L of NAA and 0.7 g/L of activated carbon for rooting culture, and 7, transplanting the culture medium to a hole tray with peat as a matrix for culturing to obtain a snail begonia plant.The method has the advantages that 30-60 plantlets can be bred after one leaf is used for 110-140 days, the breeding coefficient is greatly increased, efficient breeding of the snail begonia is promoted, large-scale production is formed as soon as possible, and market requirements are met.

Description

technical field [0001] The invention relates to a snail begonia in vitro tissue culture propagation method, which belongs to the technical field of plant breeding. Background technique [0002] Begonia is an important horticultural plant widely cultivated in the world. It is mainly divided into three categories: flower viewing, foliage viewing and flower and leaf appreciation. Among them, the foliage begonia is very popular because of its rich leaf color and unique leaf shape. Favorite, representative species - snail begonia, whose leaves are shaped like snail shells, has gradually become the new favorite of gardening enthusiasts. In the prior art, snail begonias can be propagated by leaf cuttings, but the propagation coefficient is low, and only 4-6 seedlings can be propagated from one leaf, which makes it difficult to form large-scale production in a short period of time and cannot meet market demand. Therefore, it is necessary to improve the snail begonia breeding method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 杜文文段青王继华王祥宁贾文杰马璐琳李想
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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