SNP (Single Nucleotide Polymorphism) marker site related to bovine erythrocyte membrane structure and application of SNP marker site

A technology for marking sites and cell membranes, applied in the field of genetic engineering, can solve problems such as the lack of relevant reports on the role of the SPTB gene

Pending Publication Date: 2022-04-29
FOSHAN UNIVERSITY +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many genes related to hypoxia adaptation have been discovered through the research on plateau indigenous residents and plateau animals, but there is no relevant report on the role of SPTB gene in animal hypoxia adaptation in China

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) marker site related to bovine erythrocyte membrane structure and application of SNP marker site
  • SNP (Single Nucleotide Polymorphism) marker site related to bovine erythrocyte membrane structure and application of SNP marker site
  • SNP (Single Nucleotide Polymorphism) marker site related to bovine erythrocyte membrane structure and application of SNP marker site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] In order to make the purpose, technical solutions and beneficial effects of the present invention clearer, the preferred embodiments of the present invention will be described in detail below, so as to facilitate the understanding of the skilled person.

[0023] 1. Collection of experimental animals and blood samples

[0024] In the present invention, a total of 100 Tibetan yellow cattle and Yunnan native yellow cattle distributed in 5 altitude gradients were selected for whole genome resequencing, using MEMEA, F ST Two analytical methods to screen SNPs and candidate genes for hypoxia adaptation in plateau cattle. Among the candidate genes, an elevation trend SNP was found in the SPTB gene related to bovine erythrocyte membrane structure. On the basis of screening the genes associated with hypoxia positive selection and their altitude trend loci, 139 yaks and 296 middle-altitude local cattle were selected to measure their blood physiological indicators and perform SNP ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an SNP (Single Nucleotide Polymorphism) marker site related to a bovine erythrocyte membrane structure and application thereof, and belongs to the field of gene engineering. The SNP marker is located at a G108C site (chr10: 77230398) of a 19th exon of an SPTB gene of a No.10 chromosome of cattle, the site is subjected to non-synonymous mutation (glutamic acid is changed into aspartic acid, Glu1370Asp), and the relative index of whole blood high shear of a CC genotype individual is remarkably lower than that of a GG or GC genotype individual (Plt, 0.01), so that the CC genotype has higher erythrocyte deformability; the hypoxia tolerance and plateau adaptability of CC genotype individuals are obviously higher than those of GG or GC genotype individuals. The SNP site on the SPTB gene related to the bovine erythrocyte membrane structure and hypoxia tolerance can be used as a molecular marker for bovine hypoxia tolerance or breeding plateau hypoxia environment-resistant cattle, and meanwhile, the SNP site has important significance on preservation and utilization of bovine characteristic genes.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to a SNP marking site related to bovine erythrocyte membrane structure and its application. Background technique [0002] Hereditary spherocytosis (HS) is a group of inherited disorders characterized by the presence of spherical red blood cells on peripheral blood smears and is caused by mutations in genes that regulate red blood cell membrane proteins. HS is characterized by anemia, jaundice, splenomegaly, and cholelithiasis. The mutation of SPTB gene encoding β-spectrin subunit leads to spherocytosis type 2. SPTB gene is a common pathogenic gene in HS except ANK1 gene. It is located on chromosome 14q23-q24.2 and consists of 32 exons. β-spectrin is a multifunctional protein consisting of two subunits, α and β, which, despite some similarities, are structurally distinct and encoded by different genes (Speicher et al. 1984) . α and β spectrin chains intertwine in an ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 苟潇孔小艳杨舒黎展亚楠朱莉唐琳张晓燕李昕鹏
Owner FOSHAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap