Process for preparing oat ACE (angiotensin converting enzyme) inhibitory peptide by liquid fermentation method
A technology for liquid fermentation and inhibition of peptides, applied in the fields of fermentation, peptide preparation methods, peptides, etc., can solve the problems such as the lack of research on microbial fermentation methods, and achieve the effect of solving the problems of bitterness and pigment, high fermentation efficiency and good quality.
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Embodiment 1
[0051] A process for preparing oat ACE inhibitory peptides by liquid fermentation, the preparation method comprising the following steps:
[0052] S1, strain activation
[0053] Prepare activated medium: yeast powder 0.4mg / 100mL, beef extract 4g / 100mL, peptone 2g / 100mL, NaCl 0.6g / mL, glucose 0.4g / 100mL, agar 3g / 100mL. Transfer the preserved strains to the medium, culture them in a biochemical constant temperature incubator at 38°C for 48 hours, take a single colony that has grown well and re-streak culture on the slope, transfer 2-4 times, and observe whether they are fully activated ;
[0054] S2, strain expansion
[0055] Take 100 mL of the culture medium prepared by the same method as S1, inoculate 3-4 rings of activated single colonies in the culture medium, and expand culture in a constant temperature shaking shaker at 38°C and 180r / min for 26, 28, 30, 32 , 34h;
[0056] S3, bacterial suspension preparation
[0057] Centrifuge the seed solution at 5°C and 5000Xg for 30...
Embodiment 2
[0072] A process for preparing oat ACE inhibitory peptides by liquid fermentation, the preparation method comprising the following steps:
[0073] S1, strain activation
[0074] Prepare activated medium: yeast powder 0.4mg / 100mL, beef extract 4g / 100mL, peptone 2g / 100mL, NaCl 0.6g / mL, glucose 0.4g / 100mL, agar 3g / 100mL. Transfer the preserved strains to the medium, culture them in a biochemical constant temperature incubator at 38°C for 48 hours, take a single colony that has grown well and re-streak culture on the slope, transfer 2-4 times, and observe whether they are fully activated ;
[0075] S2, strain expansion
[0076] Take 100mL of the culture medium prepared by the same method as S1, inoculate 3-4 rings of activated single colonies in the culture medium, and expand the culture in a constant temperature shaking shaker at 38°C and 180r / min for 28h;
[0077] S3, bacterial suspension preparation
[0078] Centrifuge the seed solution at 5°C and 5000Xg for 30 minutes, pou...
Embodiment 3
[0093] A process for preparing oat ACE inhibitory peptides by liquid fermentation, the preparation method comprising the following steps:
[0094] S1, strain activation
[0095] Prepare activated medium: yeast powder 0.4mg / 100mL, beef extract 4g / 100mL, peptone 2g / 100mL, NaCl 0.6g / mL, glucose 0.4g / 100mL, agar 3g / 100mL. Transfer the preserved strains to the medium, culture them in a biochemical constant temperature incubator at 38°C for 48 hours, take a single colony that has grown well and re-streak culture on the slope, transfer 2-4 times, and observe whether they are fully activated ;
[0096] S2, strain expansion
[0097] Take 100mL of the culture medium prepared by the same method as S1, inoculate 3-4 rings of activated single colonies in the culture medium, and expand the culture in a constant temperature shaking shaker at 38°C and 180r / min for 28h;
[0098] S3, bacterial suspension preparation
[0099] Centrifuge the seed solution at 5°C and 5000Xg for 30 minutes, pou...
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