Freeze-thaw meat identification model and identification method based on low-field nuclear magnetic resonance data

A low-field nuclear magnetic resonance and identification model technology, which is applied to the identification model and identification field of frozen-thawed meat, can solve the problems of long identification time, high identification cost, cumbersome identification process, etc., and achieve the effect of improving the quality control of raw meat

Pending Publication Date: 2022-05-06
南京海关动植物与食品检测中心 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have various problems, which are mainly reflected in the three aspects of cumbersome identification process, high identification cost and long identification time.

Method used

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  • Freeze-thaw meat identification model and identification method based on low-field nuclear magnetic resonance data
  • Freeze-thaw meat identification model and identification method based on low-field nuclear magnetic resonance data
  • Freeze-thaw meat identification model and identification method based on low-field nuclear magnetic resonance data

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The establishment of embodiment 1 model

[0039] Tenderloins from 9 different Landrace pigs were purchased from farmers’ markets, and 2 pieces were purchased from each pig, totaling 18 pieces. Then after removing the fat and connective tissue on the surface, keep about 500g long strips for later use.

[0040] After removing the front 1cm of meat from each piece of fresh pork tenderloin, cut a 1cm thick piece of meat, take about 4g of meat from the center, and put it in a sample bottle as a control sample. Two samples of No. 1 tenderloin were taken as parallel samples for investigation. Take 1 sample for the rest.

[0041] The remaining samples were frozen in a freezer at -18°C, taken out after three days, thawed in a freezer at 4°C for one night (12 hours) and then taken out. After removing the front 1cm meat of each tenderloin, cut a piece of 1cm thick meat Take about 4 g of meat from the center, put it in a sample bottle, and repeat the above steps for a total of 6 ...

Embodiment 2

[0080] The inspection of embodiment 2 model

[0081] Two methods of back-substitution validation and cross-validation were used to test the model, and the results are shown in Table 6.

[0082] Table 6 Classification results of discriminant studies

[0083]

[0084] It can be seen from Table 6 that the above discriminant model has a correct rate of 95.5% for the original cases, especially for the control group, there is no wrong case. At the same time, the correct rate of the case-control group is still 100% in the cross-validation, and the total correct discrimination rate in the cross-validation can reach 94.6%. The data of the two verification methods are close, which also shows that the model is stable. It can be seen that the frozen-thawed meat discrimination model constructed on the basis of low-field nuclear magnetic resonance detection data disclosed by the present invention is feasible to distinguish frozen-thawed meat, and can meet the requirements of actual dete...

Embodiment 3

[0085] Embodiment 3 detection example explanation

[0086] After removing the fat and connective tissue on the surface of the sample, cut off 1cm of meat at the front, then cut a 1cm thick piece of meat, take about 4g of meat from the center, and put it in a sample bottle for testing;

[0087] First, put the samples to be tested in a water bath at 32°C (the working temperature of the instrument) for 15 minutes before putting them into the sample tube of the instrument.

[0088] Then, put the sample to be detected into a low-field nuclear magnetic resonance instrument, and adopt the CPMG sequence detection method. Instrument parameters: radio frequency delay (RFD) = 0.08ms, pre-amp position (PRG) = 1, analog gain (RG) = 20.0db, digital gain (DRG) = 3, main frequency (SF) = 21MHz, sampling frequency (SW) = 100kHz, sampling points (TD) = 1000062, waiting time (TW) = 20000ms, echo time (TE) = 0.5ms, echo number (NECH) = 10000, accumulation times (NS) = 4.

[0089] The data proce...

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Abstract

The invention relates to the field of modern inspection and quarantine, in particular to comprehensive application of a low-field nuclear magnetic resonance detection means and a chemometrics method in the field of inspection and quarantine, and more particularly relates to a frozen-thawed meat identification model and identification method based on low-field nuclear magnetic resonance data. According to the method, a low-field nuclear magnetic resonance technology and a chemometrics method are organically combined, so that a discrimination model for effectively discriminating fresh meat, slightly frozen and thawed meat and frozen and thawed meat is obtained, the correct discrimination rate of the model can reach 94.6% through verification, and the method can be used as a model and a method for discriminating the frozen and thawed meat. Therefore, a new thought and a new method are provided for rapidly detecting the frozen and thawed meat, and the method has important significance for improving the quality control of raw meat in China.

Description

technical field [0001] The present invention relates to the field of modern inspection and quarantine, in particular to the comprehensive application of low-field nuclear magnetic resonance detection means and chemometrics methods in the field of inspection and quarantine, and more specifically to the identification model of frozen-thawed meat based on low-field nuclear magnetic resonance data and Identification method. Background technique [0002] As a meat food with high protein and high nutritional value, pork has always occupied a dominant position in the meat consumption structure of Chinese residents. The quality of pork has always been the focus of consumers' attention. Compared with frozen and thawed meat, fresh meat has more market value. Therefore, in order to make huge profits, thawed meat is sold as cold fresh meat in the market, which is a bad phenomenon of shoddy. Using frozen and thawed meat as cold fresh meat not only reduces the taste of the meat and dama...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G06F17/10
CPCG01N24/08G06F17/10
Inventor 季美泉林宏丁涛费晓庆徐瑞平刘芸张晓燕陈磊殷耀王艳李贤良韩芳邓晓军郭思言苏姗姗
Owner 南京海关动植物与食品检测中心
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