Linear scanning type fluorescence detection system based on no optical fiber and PCR (Polymerase Chain Reaction) instrument

Abstract

The invention discloses an optical fiber-free linear scanning type fluorescence detection system. The system comprises a PCR instrument; belongs to the field of medical inspection instruments and molecular diagnosis detection instruments. The technical key points are as follows: the fluorescence detection assembly and the reaction tank assembly are respectively arranged on two sides of the vertical fixing plate; light through holes in the pipe wall of the reaction tank are in one-to-one correspondence with light through holes formed in the vertical fixing plate; a light communication port of the fluorescence detection module and a light through hole formed in the vertical fixing plate are kept at the same height, and the fluorescence detection module adopts an exciting light-receiving light integrated design (namely, the exciting light-receiving light passes through one light communication port); the fluorescence detection assembly can reciprocate in the Y direction, and all the reaction tanks can be detected through linear scanning. The invention aims to provide the optical fiber-free linear scanning type fluorescence detection system and the PCR instrument, so that the small size of the PCR instrument can be realized, and the manufacturing cost and the use cost can be reduced.

Description

technical field [0001] The present invention relates to the field of a medical inspection instrument and a molecular diagnosis instrument, and more specifically relates to a linear scanning fluorescence detection system based on no optical fiber and a PCR instrument. Background technique [0002] Real-time fluorescence quantitative PCR detection system, its English abbreviation is: real-time quantitative PCR detection system, since it was proposed in 1993, it has been widely used in technical fields such as disease detection, inspection and quarantine, and genetic engineering research. [0003] At present, common real-time fluorescent quantitative PCR detection systems mainly include: 96-well plate fluorescent real-time quantitative PCR system (such as reference 1: Wang Weiping. Development of 96-well real-time fluorescent quantitative PCR detection system [D]. Zhejiang University of Technology, 2011) Centrifugal structure (such as reference 2: Wang Haiqing, Wang Yi, Shi Wuh...

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Problems solved by technology

[0004]However, if the optical fiber can be eliminated, the volume of the instrument can be further reduced

However, this technical idea is contrary to the existing technology

The applicant also searched databases such as Himmpat, EPO, and CNKI: "no optical fiber and fluorescence andPCR", and the retrieval results were all 0, which proves that at present, there is no PCR fluorescence detection system without optical fiber

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