Method for improving fluorescence imaging definition
A fluorescence imaging and definition technology, applied in image enhancement, image analysis, image data processing, etc., can solve the problem of lack of high definition, and achieve the effect of reducing complicated calculations and high definition
Pending Publication Date: 2022-05-10
NANJING NUOYUAN MEDICAL DEVICES CO LTD
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[0004] At present, the analysis of plant stress has far-reaching significance for agricultural and environmental ecological research. Polarization imaging and plant chlorophyll fluorescence imaging detection also have the advantages of non-invasive and non-destructive. Therefore, it is a good method to study and analyze plant stress through fluorescence imaging technology. However, there is no high definition. The existing technology pays more attention to research and biological cells. Therefore, in the study of plant stress for agricultural and environmental ecological research, people pay little attention. Fluorescence imaging is still far from ultra-high-definition. , which also brings great challenges to the work of plant stress researchers
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[0048] refer to figure 1 and figure 2 , which is the first embodiment of the present invention, provides an implementation of a method for improving the clarity of fluorescence imaging, specifically including:
[0049] S1: Obtain the smoothed power spectrum of the received imaging signal, and calculate the resolution. It should be noted that the calculation definition includes:
[0050] Using the index L-Z complexity strategy to describe the sequence characteristics, the received imaging signal is smoothed to obtain the power spectrum;
[0051] Define {s(k)} as the amplitude spectrum of the signal, k=1,2,...,N, N as the signal data length, and quantize and encode {s(k)};
[0052] Set the number of quantization levels to L, let a=max{s(k)}, which represents the maximum value of the signal amplitude spectrum, and divide {s(k)} into L layers in the interval of (0, a], then:
[0053]
[0054] Among them, {r(k)} represents {s(k)} digit sequence with L symbols after quantiza...
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The invention discloses a method for improving the definition of fluorescence imaging, which comprises the following steps of: solving a power spectrum for smoothing a received imaging signal, and calculating the definition; according to the definition, setting a threshold value to identify a conventional imaging signal, and if the conventional imaging signal cannot be identified, searching a smoothed power spectrum peak; if the spectrum peak is 2, 2FSK is determined, and if the spectrum peak is not 2, squaring processing is carried out on the conventional imaging signal and smoothing processing is carried out on the power spectrum; and fitting the instantaneous frequency by using a least square method, carrying out local de-noising, and outputting to obtain an imaging signal with relatively high definition. According to the method disclosed by the invention, a better frequency signal is obtained by performing special processing on a fluorescence imaging signal, namely, higher definition is obtained, complicated operation is reduced, and the method can be well applied to plant stress analysis and research.
Description
technical field [0001] The invention relates to the technical fields of plant stress analysis and fluorescence imaging clarity, in particular to a method for improving the fluorescence imaging clarity. Background technique [0002] Fluorescence is a common luminescent phenomenon in nature. Fluorescence is produced by the interaction between photons and molecules. This interaction process can be described by the Jablonski (Jablonslc) molecular energy level diagram: most molecules are in the normal state. The lowest vibrational energy level So of the ground state, when excited by energy (light energy, electrical energy, chemical energy, etc.), the electrons around the nucleus transition from the ground state energy level So to a higher energy excited state (the first or second excited state ), the electrons in the excited state are in a high-energy state, unstable, and will release energy back to the ground state through two ways, one is the radiative transition that releases ...
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IPC IPC(8): G06T5/00G06V10/762G06K9/62
CPCG06T2207/10064G06F18/2321G06T5/73G06T5/70
Inventor 蔡惠明李长流朱淳潘洁
Owner NANJING NUOYUAN MEDICAL DEVICES CO LTD