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Application of SLC12A3 and/or SLC12A9 as detection index for treatment and prognosis of uveal melanoma

A melanoma, uveal technology, applied in the application field of uveal melanoma treatment and prognosis detection indicators, can solve the problem that primary uveal melanoma cannot develop, uveal melanoma has no change in distant metastasis rate and mortality And other issues

Pending Publication Date: 2022-05-13
THE EYE HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite improvements in local therapy, including surgery and radiation therapy, rates of distant metastasis and mortality from uveal melanoma have remained unchanged over the past decade, suggesting that current treatments for primary uveal melanoma are not effective. better prognosis

Method used

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  • Application of SLC12A3 and/or SLC12A9 as detection index for treatment and prognosis of uveal melanoma
  • Application of SLC12A3 and/or SLC12A9 as detection index for treatment and prognosis of uveal melanoma
  • Application of SLC12A3 and/or SLC12A9 as detection index for treatment and prognosis of uveal melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 Correlation between the expression levels of SLC12A3 and SLC12A9 and the prognosis of UVM patients

[0088] In order to evaluate the relationship between SLC12s and the prognosis of UVM patients, the present invention carried out univariate Cox regression analysis in the TCGA-UVM cohort. The results showed that high expression of SLC12A3 and SLC12A9 was significantly associated with poorer overall survival (OS) (SLC12A3: HR=1.16, 95%CI=1.03-1.29, P=0.01; SLC12A9: HR=2.36, 95%CI= 1.14-4.88, P=0.02), significantly correlated with disease-specific survival (DSS) (SLC12A3: HR=1.16, 95% CI=1.03-1.31, P=0.01; SLC12A9: HR=2.55,95 %CI=1.20-5.45, P=0.02), while other SLC12 family members did not show significant correlation (such as figure 1 shown in A).

[0089] Survival analysis also showed that according to the expression levels of SLC12A3 and SLC12A9, 77 UVM patients could be divided into high-risk group and low-risk group with significant differences in OS and D...

Embodiment 2

[0091] Example 2 Effect of Overexpression of SLC12A3 and / or SLC12A9 on the Proliferation of Uveal Melanoma Cells

[0092] The present invention analyzes the expression of SLC12A3 and SLC12A9 in uveal melanoma tissue by means of immunohistochemistry. Immunohistochemical staining showed that SLC12A3 and SLC12A9 were lowly expressed in normal retinal paracancerous tissues and highly expressed in uveal melanoma tissues (eg figure 2 shown in A-C). In order to study the functional role of SLC12A3 and SLC12A9 in uveal melanoma, the present invention uses lentivirus infection to overexpress SLC12A3 and SLC12A9 in uveal melanoma cell lines C918 and MUM2C. The results of Western blotting showed that the protein levels of SLC12A3 and SLC12A9 were increased in the overexpressed cell lines (such as figure 2 D and figure 2 E shown). Cell growth curves showed that overexpression of SLC12A3 and SLC12A9 both increased cell proliferation activity compared with the EGFP control group (eg ...

Embodiment 3

[0093] Example 3 Effect of Knockdown of SLC12A3 and SLC12A9 on the Proliferation of Uveal Melanoma Cells

[0094] The present invention knocks down SLC12A3 or SLC12A9 in uveal melanoma cell lines, or simultaneously knocks down SLC12A3 and SLC12A9, and finds that si-SLC12A3 (si-A3 for short) and si-SLC12A9 (si-A9 for short) can effectively Knockdown of SLC12A3 and SLC12A9 (eg image 3 shown in A). Cell growth curves showed that knocking down SLC12A3 and SLC12A9 could inhibit the proliferation of C918 cells (such as image 3 shown in B). Simultaneously knocking down SLC12A3 and SLC12A9 in C918 cells had a stronger inhibitory effect on cell proliferation (eg image 3 shown in B). In order to further confirm this result in other uveal melanoma cell lines, the present invention also knocked down SLC12A3 and SLC12A9 in MUM2C cells, and the cell growth curve also showed that the proliferation of cells knocked down by SLC12A3 and SLC12A9 was inhibited ( image 3C). Consistent wi...

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Abstract

The invention discloses an application of SLC12A3 and / or SLC12A9 as an index for treatment and prognosis detection of uveal melanoma. The invention further discloses a calculation model for predicting uveal melanoma prognosis and a medicine for preventing or treating uveal melanoma. The invention provides a new method for prognosis monitoring and treatment of uveal melanoma patients.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of SLC12A3 and / or SLC12A9 as indicators for the treatment and prognosis of uveal melanoma. Background technique [0002] Uveal melanoma (Uveal Melanoma, UVM) is the most common primary malignant tumor in the adult eye. The age of onset is mostly in people over 50-60 years old, and male patients are slightly more common. The incidence rate of uveal melanoma abroad is 0.02%. In China, uveal melanoma is the second most common intraocular malignant tumor. Uveal melanoma can occur anywhere in the uvea, but the choroid is the most common. It is usually unilateral, but rare in both eyes. Uveal melanoma cells can metastasize to distant places along with the blood line in the early stage, even before the primary ocular tumor is discovered, the distant metastasis has already begun. The metastases of uveal melanoma are mainly through the blood, and the most common me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00G16C20/50
CPCC12Q1/6886G01N33/5743G01N33/57484A61K45/00A61P35/00G16C20/50C12Q2600/106C12Q2600/158G01N2333/47
Inventor 周猛闫聪聪孙杰郑钦象麻晓银
Owner THE EYE HOSPITAL OF WENZHOU MEDICAL UNIV
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