DcBGLU2L dendrobium polysaccharide content molecular marker as well as development method and application thereof

Abstract

The invention discloses a DcBGLU2L dendrobium polysaccharide content molecular marker as well as a development method and application thereof. According to the invention, two common plant hormones NAA and 6-BA in orchid tissue culture are adopted to treat dendrobium officinale seedlings, comparative analysis is carried out on main medicinal compounds in the dendrobium officinale seedlings, and transcriptome sequencing is carried out on corresponding samples to analyze differential expression genes. The corresponding relation between compound accumulation and differential gene expression modes under different treatment conditions is defined. Wherein the expression of the DcBGLU2L gene is closely related to the polysaccharide content, and the expression level of the DcBGLU2L gene can indicate the polysaccharide accumulation level, so that the DcBGLU2L gene can be used as a molecular marker for indicating the polysaccharide content; the method can be applied to production practice to monitor the polysaccharide content in acceptor materials treated by different hormones, with different concentrations, at different treatment times and in different growth periods; and a way is laid for manually controlling the production of the active ingredients of the dendrobium nobile.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a DcBGLU2L dendrobium polysaccharide content molecular marker and its development method and application. Background technique [0002] Dendrobium is one of the largest genera in Orchidaceae, including more than 1,500 species worldwide, and has high economic and scientific research value. Dendrobium officinale (D. officinale) is an endangered perennial epiphytic herb widely distributed in subtropical Asia. It is listed as one of the most precious plants of the genus Dendrobium. effect. Its stem contains a variety of active ingredients, mainly including polysaccharides, alkaloids, flavonoids, phenols, etc. [0003] Dendrobium candidum is widely used in medicines and dietary supplements in the form of juice, powder, capsules, and functional wine or tea according to consumer preference, leading to increasing demand for this species and intensification of agricultural production. Acco...

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Problems solved by technology

Therefore, the quality of Dendrobium candidum production is of paramount importance, and it can be affected by various genetic and environmental factors, resulting in unstable content of active ingredients

[0004] At present, the medium widely used in the rapid propagation system of Dendrobium officinale induces seed germination, resulting in unstable phenotypic traits and medicinal quality of offspring

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