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Novel flavonoids of Luo Han Guo, its preparation method and application in honeybee attractant

A technology of flavonoids and compounds, applied in botany equipment and methods, attracting pests, organic chemical methods, etc., can solve the problems of polluting the environment, wasting resources, and not being effectively used, and achieves simple preparation methods and easy operation Easy and good effect of attracting activity

Active Publication Date: 2022-07-26
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the planting process of Luo Han Guo, a large amount of by-product resources such as Luo Han Guo stems, leaves, and rattans are produced, but they have not been effectively utilized. Although the leaves are used as tea in the folk, they are generally discarded as waste, which is a waste of resources and pollution. surroundings

Method used

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  • Novel flavonoids of Luo Han Guo, its preparation method and application in honeybee attractant
  • Novel flavonoids of Luo Han Guo, its preparation method and application in honeybee attractant
  • Novel flavonoids of Luo Han Guo, its preparation method and application in honeybee attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of the new flavonoid XY8:

[0049] 1) Obtain the leaf extract of Luo Han Guo male plant

[0050] Weigh 1Kg of dried leaves of the male leaves of Luo Han Guo (Siraitia grosvenorii (Swingle) C. Jeffrey), pulverize, add 8 times the weight of 70% (volume) methanol, heat under reflux at 60°C for 60 min, filter, and collect the filtrate. The filter residue was added with 8 times the weight of 70% (volume) methanol again, heated under reflux at 60° C. for 60 min to extract, and filtered. The filtrates were combined, and the filtrate was concentrated to 2L by evaporation under reduced pressure with a rotary evaporator at 60°C. Add 2L of methanol to the concentrated solution, extract twice with petroleum ether, and use 2L each time to collect the lower phase. The petroleum ether phase recovers the solvent and discards it.

[0051] 2) Preliminary purification of the extract

[0052] Take the extract LHGXY01 and put it on the D101 macroporous adsorption resin column...

Embodiment 2

[0067] Preparation of the new flavonoid XY8:

[0068] 1) Obtain the leaf extract of Luo Han Guo male plant

[0069] Weigh 1Kg of dried leaves of the male plant leaves of Luo Han Guo (Siraitia grosvenorii (Swingle) C. Jeffrey), pulverize, add 8 times the weight of water, heat under reflux at 50°C for 60 min, filter, and collect the filtrate. The filter residue was added with 8 times the weight of water again, heated and refluxed at 50°C for 60 min to extract, and filtered. The filtrates were combined, and the filtrate was concentrated to 2L by evaporation under reduced pressure with a rotary evaporator at 60°C. Add 2L of methanol to the concentrated solution, extract twice with petroleum ether, and use 2L each time to collect the lower phase. The petroleum ether phase recovers the solvent and discards it.

[0070] Steps 2) to 3) are the same as in Example 1.

[0071]Step 3) of the present embodiment is separated by high-speed countercurrent chromatography, and the obtained f...

Embodiment 3

[0073] Preparation of the new flavonoid XY8:

[0074] 1) Obtain the leaf extract of Luo Han Guo male plant

[0075] Weigh 1Kg of dried leaves of the male plant leaves of Luo Han Guo (Siraitia grosvenorii (Swingle) C. Jeffrey), pulverize, add 8 times the weight of 100% (volume) ethanol, heat under reflux at 40° C. for extraction for 60 min, filter, and collect the filtrate. Add 8 times the weight of 100% (volume) ethanol to the filter residue again, extract by heating under reflux at 40° C. for 60 min, and filter. The filtrates were combined, and the filtrate was concentrated to 2L by evaporation under reduced pressure with a rotary evaporator at 60°C. Add 2L of methanol to the concentrated solution, extract twice with petroleum ether, and use 2L each time to collect the lower phase. The petroleum ether phase recovers the solvent and discards it.

[0076] 2) High-speed countercurrent chromatographic separation

[0077] 2.1) Measure 600 mL of butyl acetate, 200 mL of acetone,...

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Abstract

The invention discloses a new flavonoid compound of Luo Han Guo, a preparation method thereof and an application in a honeybee attractant, belonging to the technical field of new flavonoid compounds; the compound is a compound having the following structure or a pharmaceutically acceptable salt thereof: The leaves of the male Luo Han Guo are extracted, the obtained extract is separated by high-speed countercurrent chromatography, the fractions are collected in sections, identified by UPLC‑MS or TLC, and the fractions are combined, and the solvent is recovered to obtain a total of 6 fractions I to VI, respectively. The yellow powdery counter-current chromatographic purified product in Part IV is the new flavonoid compound with the structure shown in the above formula; the new flavonoid compound of the present invention, derived from Luo Han Guo, is a new natural compound, which has better properties for honeybees. The attracting activity of the herb provides an ideal candidate compound for the development of a new type of bee attractant, which not only opens up a new bee attractant, but also opens up a new application field of Luo Han Guo, which lays the foundation for the further development and utilization of Luo Han Guo leaves.

Description

technical field [0001] The invention belongs to the technical field of new flavonoids, in particular to a new flavonoid of Luo Han Guo, a preparation method thereof and an application in a honeybee attractant. Background technique [0002] Flavonoids refer to two benzene rings (A ring and B ring) connected to each other through the central three carbon atoms, with C 6 -C 3 -C 6 A series of compounds of the backbone, widely distributed, mainly exist in the form of glycosides in plants, and play an important role in plant growth, development, development, fruiting, and defense against pathogens and animals. At the same time, flavonoids are also an important class of biologically active ingredients, which have various physiological functions such as regulating immunity, improving blood circulation, anti-virus, anti-inflammatory, anti-tumor, anti-aging, protecting liver and kidney, lowering blood sugar, and lowering blood lipids. pharmacological effects. New flavonoids not o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H17/08A01N43/16A01P19/00
CPCC07H17/07C07H17/08A01N43/16C07B2200/07Y02A50/30C07H1/08A01P19/00
Inventor 胡兴华霍华珍叶家桐谢运昌朱晓珍邓涛蔡爱华李典鹏
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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