Method for rapidly breeding seedlings by using alpinia katsumadai stem tips

A cardamom and stem tip technology, applied in the field of agricultural biology, achieves the effects of orderly growth, promotion of development and utilization, protection of wild resources, and consistent physiological age

Pending Publication Date: 2022-06-03
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for rapid breeding of seedlings using the stem tip of Cardamom grassa, using the stem tip of the excellent plant of Cardamom grass as an explant, and multiplying by direct differentiation of adventitious buds from the shoot tip, which can be used in a short time Batches of cardamom tissue culture seedlings with consistent traits and neat growth are obtained in batches, which are suitable for industrial seedling breeding and large-scale cultivation, solve the demand for excellent seedlings in artificial planting, and play an important role in promoting the development and utilization of cardamom and the protection of wild resources

Method used

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  • Method for rapidly breeding seedlings by using alpinia katsumadai stem tips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. The process of shoot apex-induced differentiation of adventitious buds

[0027] Take the unflowered tiller of cardamom, clean and sterilize, dry the water with sterile paper, peel off the tip of the rice grain size (0.5-1mm), and inoculate it in the induction medium (WT+6-BA 6.0mg / L+NAA) 0.9mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8) to induce differentiated adventitious buds. Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol m -2 ·s -1 . After about 30 days of culture, adventitious buds began to differentiate.

[0028] 2. Proliferation and culture process

[0029] The adventitious buds were transferred to proliferation medium (WT+6-BA 3.0mg / L+NAA 0.5mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8) for subculture to form cluster buds. The culture conditions were the same as in step 1.

[0030] 3. Induce rooting process

[0031] When the clump buds grow to a height of 3 to 4 cm, cut out individual pla...

Embodiment 2

[0039] 1. The process of shoot apex-induced differentiation of adventitious buds

[0040] Take the sprouts of cardamom shoots, dry them with sterile paper after cleaning and disinfection, peel off the shoot tips of the size of rice grains (0.5-1mm), and inoculate them in the induction medium (WT+6-BA 10.0mg / L+NAA 0.6mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8) induced differentiated adventitious buds. Culture conditions: culture temperature 24 ~ 26 ℃, light culture. Lighting time 10~12h / d, light intensity 20~30μmol·m -2 ·s -1 . After about 30 days of culture, adventitious buds began to differentiate.

[0041] 2. Proliferation and culture process

[0042] The adventitious buds were transferred to proliferation medium (WT+6-BA 5.0mg / L+NAA 0.2mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8) for subculture to form cluster buds. The culture conditions were the same as in step 1.

[0043] 3. Induce rooting process

[0044]When the clump buds grow to a height of 3-4cm, cut o...

Embodiment 3

[0052] 1. The process of shoot apex-induced differentiation of adventitious buds

[0053] Take the unflowered tiller of cardamom, clean and sterilize it, dry up the water with sterile paper, peel off the tip of the rice grain size (0.5-1mm), and inoculate it in the induction medium (WT+6-BA 8.0mg / L+NAA) 0.7mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8) to induce differentiated adventitious buds. Culture conditions: culture temperature 24 ~ 26 ℃, light culture. Lighting time 10~12h / d, light intensity 20~30μmol·m -2 ·s -1 . After about 30 days of culture, adventitious buds began to differentiate.

[0054] 2. Proliferation and culture process

[0055] The adventitious buds were transferred to proliferation medium (WT+6-BA 4.0mg / L+NAA 0.4mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8) for subculture to form cluster buds. The culture conditions were the same as in step 1.

[0056] 3. Induce rooting process

[0057] When the clump buds grow to a height of 3-4cm, cut out a sin...

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Abstract

The invention belongs to the technical field of agricultural biology, and relates to a method for rapidly breeding seedlings by using alpinia katsumadai stem tips, which comprises the processes of induced differentiation of adventitious buds, subculture multiplication, rooting induction, transplanting and management. According to the method, the technological process is simple, the adventitious buds are directly induced and differentiated by taking bamboo shoot buds or stem tips of non-flowering tillering plants as explants, the adventitious buds are subjected to subculture multiplication to form clumpy buds, the adventitious buds are rooted and transplanted, a large number of healthy and high-quality seedlings capable of being used for production and cultivation can be obtained in a short time, the period is short, the obtained seedlings are consistent in physiological age, and the yield is high. The alpinia katsumadai seedlings grow neatly, are suitable for industrialized seedling production and large-scale cultivation, meet the requirements of artificial planting for excellent seedlings, and play an important role in promoting development and utilization of alpinia katsumadai and protecting wild resources.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and relates to a method for breeding seedlings by tissue culture of Cardamom, in particular to a method for rapidly breeding seedlings by using the shoot tips of Cardamom. The method utilizes the shoot tips of cardamom to directly induce differentiated adventitious buds, and obtains a large number of seedlings through processes such as sub-generation proliferation, rooting induction, and transplanting of adventitious buds. Background technique [0002] Alpinia katsumadai Hayata, also known as cardamom, leucorrhea, grass fruit, cardamom, grass cardamom, large grass cardamom, even child, grass cardamom, Fei Leizi, bentzi, etc., is a perennial herb of the ginger family Alpinia , cultivated in Hainan, Yunnan and Guangxi, China. Its dried seeds are used as medicine and have high medicinal value and development prospect. [0003] The key to large-scale artificial planting of cardamom is to c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02P60/40
Inventor 李志英符运柳徐立
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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