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Application of human chromosome InDel gene locus, primer group and product thereof, and individual identification method of detected material

An identification method and locus technology, which can be used in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., and can solve problems such as low efficacy of paternity testing

Active Publication Date: 2022-06-03
ACADEMY OF MILITARY MEDICAL SCI
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Problems solved by technology

Although the identification efficiency of 47 InDels is greater than the TDP (0.999 999 999 98) of the conventional STR system, which can meet the needs of individual identification, but CPEtrio<0.999 9, the efficacy of paternity identification is low and needs to be further improved

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  • Application of human chromosome InDel gene locus, primer group and product thereof, and individual identification method of detected material
  • Application of human chromosome InDel gene locus, primer group and product thereof, and individual identification method of detected material
  • Application of human chromosome InDel gene locus, primer group and product thereof, and individual identification method of detected material

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Experimental program
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Embodiment 1

[0063] 1 Materials and methods

[0064] 1.1 InDel Screening Process

[0065] In this study, based on the dbSNP database (https: / / www.ncbi.nlm.nih.gov / snp / ) with human GRCh37.hg19 as the reference genome, VCFtools and plink software were used to screen out those with high identification in the Chinese population. Potency of the InDel locus. The specific screening criteria are as follows: (1) Binary polymorphic insertion / deletion sequences located in the autosomal intron region with a global minor allele frequency (GMAF) between 0.4 and 0.5, and obtained 17374 (2) Satisfy Hardy-Weinberg Equilibrium (HWE) law, P HWE >0.05, heterozygosity >0.4, 1263 loci were obtained; (3) the linkage equilibrium test (LE) was met, r 2 1bp, 172 loci were obtained; (6) On the same chromosome, the physical distance between InDel loci was ≥10MB, and 93 loci were obtained. Primer Premier 5.0 and MFEprimer software were used to design multiple primers for the 93 loci obtained from the above scree...

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Abstract

The invention provides application of a human chromosome InDel gene locus, a primer group and a product thereof, and an individual identification method of a detected material, and relates to the technical field of biology. The invention provides application of 66 InDel gene loci of a human chromosome in individual identification or paternity identification. The inventor autonomously screens 66 InDel gene loci from a dbSNP database, evaluates population genetic parameters of 66-plex InDels (66 InDel gene loci) in 251 Chinese Han population, and analyzes the forensic medicine application value of the 66-plex InDels (66 InDel gene loci). Results show that 66 InDel gene loci have good genetic polymorphism in Chinese Han people, and can be independently applied to forensic individual identification and paternity identification research.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an application of a human chromosome InDel locus, a primer set, a product thereof, and an individual identification method for a test material. Background technique [0002] Short tandem repeats (STRs) have been widely used in individual identification and paternity testing due to their high sensitivity and strong discrimination ability. But the STR mutation rate is relatively high, about 10 -3 , there may be misjudgment in paternity testing; and the fragment is long (100-400bp), and the detection success rate in degraded samples is low. As a novel genetic marker, insertion / deletion polymorphism (InDel) is widely distributed in the human genome; the mutation rate is significantly lower than that of STR, about 10 -8 ; Shorter amplicon (<200bp) is more suitable for the detection of highly degraded samples; its two-state structure is easier to type and detect. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 王升启张庆珍周喆王雪倩
Owner ACADEMY OF MILITARY MEDICAL SCI
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