Glucose sensor, glucose dehydrogenase and preparation method of glucose dehydrogenase

Abstract

The invention discloses a glucose sensor, glucose dehydrogenase and a preparation method of the glucose dehydrogenase. The preparation method comprises the following steps: A) carrying out prosthetic group removal and modification on the glucose dehydrogenase; and B) recombination of glucose dehydrogenase. According to the present invention, the glucose dehydrogenase is accurately modified, and the electron transfer channel is constructed from the active center of the glucose dehydrogenase to the surface of the glucose dehydrogenase so as to achieve the direct electrochemical treatment of the glucose dehydrogenase, such that the restriction of oxygen on the glucose detection is fundamentally eliminated, and the accuracy of the glucose dynamic detection is improved.

Description

technical field [0001] The invention relates to a glucose sensor, a glucose dehydrogenase and a preparation method thereof. Background technique [0002] Diabetes is one of the three chronic diseases that threaten human health and life. Its typical clinical symptom is high blood sugar in the patient's body. Long-term high blood sugar will cause various complications and even threaten the life of the patient. Although there is currently no cure for diabetes, controlling blood sugar within the normal range as much as possible can effectively reduce and delay the occurrence of diabetic complications. Therefore, for diabetic patients, the monitoring and regulation of blood sugar has become a part of life. The implantable continuous glucose monitoring system that has emerged in recent years has provided thousands of diabetic patients with a comprehensive tool for regulating blood sugar. [0003] The working principle of the existing implantable continuous glucose monitoring sys...

AI Technical Summary

Problems solved by technology

They either have "oxygen starvation" issues, such as reliance on interstitial fluid oxygen for glucose monitoring (such as Medtronic's Guardian and Dexcom's Dexcom G5 and 6); or potential oxygen interference problems (such as Abbott's Libre and libre 2)

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