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Glucose biosensor

A biosensor and glucose technology, applied in the field of medical diagnosis, can solve the problems that the performance cannot meet the needs of continuous glucose monitoring and the working life is only one week, and achieve the effect of improving sensitivity, simplifying design and fabrication, and simplifying design and fabrication.

Pending Publication Date: 2022-03-08
SINOCARE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the highly hydrophobic requirement has brought great challenges to the design of biocompatible membranes. Although after more than 20 years of research and exploration, its performance is far from meeting the needs of continuous glucose monitoring.
For example, Medtronic's Guardian and iPro2 also need to be calibrated twice a day, and their working life is only one week

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Chemical Modification of Glucose Dehydrogenase

[0035] Incubate 1-10 mg / ml of glucose dehydrogenase (optimal value: 5) in PBS buffer solution containing 1-6 mol / L urea (optimal value: 3) at 4 degrees for 8-24 hours (optimum Value: 12), fully expand the glucose dehydrogenase. Then 1-10 mg / ml (best value: 5) of ruthenium or osmium complexes with free amino groups are fully mixed with 1-10 mg / ml glucose dehydrogenase (best value: 5), Then add 0.2-10 mmol / L carbodiimide (optimum value: 1) and 0.1-1 mmol / L N-hydroxysuccinimide (optimum value: 0.5) in sequence, after mixing thoroughly, React at 4 degrees for 8-24 hours (best value: 12). Then, the modified glucose dehydrogenase was separated and purified by ultrafiltration bag dialysis (cutting molecular weight: 1000-30000, optimal value: 10000). The purified glucose dehydrogenase can directly exchange electrons effectively with the electrode;

[0036] To further improve the rapid electron exchange between gluc...

Embodiment 2

[0037] Example 2: Preparation of Glucose Sensing Membrane

[0038] 1. Preparation

[0039] Mix 2-20 mg / ml (best value: 5) modified glucose dehydrogenase in PBS buffer solution with 0.1-5% (best value: 1) glutaraldehyde solution for 30-180 minutes Finally (optimum value: 60), the chemically cross-linked glucose dehydrogenase is coated on the surface of the electrode to form a glucose sensing film by the drop coating method or the dipping and pulling method.

[0040] 2. Characterization of electrochemical activity

[0041] The glucose sensing membrane containing modified glucose dehydrogenase was characterized by cyclic voltammetry, and the results are shown in figure 2 . figure 2 Curve a clearly shows that after the above treatment, the redox small molecule with superior electrochemical performance has been successfully bonded to glucose dehydrogenase. When 5 mmol / L glucose was added to the PBS buffer solution, the cyclic voltammogram of the glucose sensing membrane clear...

Embodiment 3

[0042] Example 3: Preparation of biocompatible membrane and glucose biosensor

[0043] 1. Preparation of biocompatible membrane

[0044] (1) Synthesis of polyhydroxyethyl methacrylate: 5-500 milliliters of hydroxyethyl methacrylate (best value: 100)+10-1000 milliliters of dehydrated alcohol (best value: 300) and 1- 100ml (best value: 30) of water, deoxygenated with argon for 10-60 minutes (best value: 30). Then add 10-1000 mg (best value: 200) of Na 2 S 2 o 8 , placed in an airtight container, and reacted at 50-75°C (best value: 70) for 10-40 hours (best value: 24). Then add 500-5000 milliliters of acetone (best value: 2500) to precipitate polyhydroxyethyl methacrylate, and centrifuge. Add ethanol to dissolve, then add 500-5000 ml (best value: 2500) acetone to precipitate, and centrifuge. Repeat several times, and finally dry the precipitate at 60-120°C (best value: 100) in vacuum for at least 8-24 hours (best value: 12).

[0045](2) Coating of polyhydroxyethyl methacry...

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Abstract

The invention relates to the technical field of medical diagnosis, and discloses a glucose biosensor. The biosensor comprises a base body and an electrode located on the base body, the electrode is coated with a glucose sensing film; the glucose sensing membrane is formed by cross-linking glucose dehydrogenase, wherein the glucose dehydrogenase is coupled with a ruthenium or osmium complex with free carboxyl or amino on the surface of a glucose dehydrogenase molecule and at the free carboxyl and amino in the glucose dehydrogenase molecule. The glucose dehydrogenase is chemically modified, so that the glucose dehydrogenase can directly exchange electrons with an electrode, and the glucose sensing film prepared on the basis of the glucose dehydrogenase is applied to a glucose biosensor, so that the restriction of oxygen on glucose detection can be fundamentally eliminated; the sensitivity, the accuracy, the reproducibility, the stability, the specificity and the anti-interference capability of glucose dynamic detection are improved, and the service life of the implantable continuous glucose monitoring system is prolonged.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to a glucose biosensor. Background technique [0002] With the development of the economy and the improvement of life, more and more people in the world have hyperglycemia or suffer from diabetes. Diabetes has become a common disease worldwide. According to statistics from the International Diabetes Federation, there are more than 400 million diabetics in the world. Among them, the number of diabetic patients in China exceeds 100 million. Diabetes is a chronic disease. Due to the decline of pancreas function in diabetic patients, it cannot produce enough insulin or can no longer produce insulin to effectively regulate blood sugar, resulting in high blood sugar in diabetic patients. Long-term high blood sugar can cause irreparable damage to human organs, causing complications such as skin ulcers, blindness, kidney failure and cardiovascular disease. Although the existing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/3277G01N27/308G01N27/48
Inventor 高志强
Owner SINOCARE
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