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KASP molecular marker related to wheat grain length and application

A wheat grain and wheat technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of large-scale screening of difficult-to-breed offspring materials, long detection cycle, high cost, etc.

Pending Publication Date: 2022-06-24
河北省农林科学院粮油作物研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used types of molecular markers include RFLP, AFLP, DArT, SSR, etc., but these marker types have a long detection cycle, cumbersome steps, and high costs, making it difficult to carry out large-scale screening of breeding offspring materials

Method used

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  • KASP molecular marker related to wheat grain length and application
  • KASP molecular marker related to wheat grain length and application
  • KASP molecular marker related to wheat grain length and application

Examples

Experimental program
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Embodiment 1

[0042] Common wheat QTL qGL3B.1 exists at the physical position 785,432,286bp of chromosome 3B (chip marker site AX-110418888, reference wheat variety Chinese spring genome IWGSC_RefSeq_v1.0, http: / / 202.194.139.32 / jbrowse-1.12.3-release / ) A SNP site is the nucleotide at position 178 of SEQ ID No. 1, and its nucleotide type is G or A, represented by the letter R. One of its allelic genotypes is GG (that is, the homozygous type with the nucleotide at position 178 of SEQ ID No. 1 being G), and this type of wheat is regarded as qGL3B.1a (also known as the G base type); the other one, etc. The allelic type is AA (that is, the homozygous type with the nucleotide at position 178 of SEQ ID No. 1 being A), and this type of wheat is referred to as qGL3B.1b (also known as the A base type).

[0043] Batch detection of qGL3B.1 allele types of common wheat varieties in each group using KASP markers was divided into the following two steps: PCR amplification and genotyping.

[0044] (1) PCR...

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Abstract

The invention discloses application of a KASP molecular marker related to wheat grain length. One technical scheme protected by the invention is application of a composition for detecting polymorphism or genotype (namely allele) of a QTLqGL3B.1 site in a wheat genome in preparation of a product for identifying or assisting in identifying grain length. The composition for detecting the polymorphism and genotype of the QTLqGL3B.1 site can be combined with other substances (such as substances for detecting the single nucleotide polymorphism or genotype of other molecular markers related to the length of wheat grains) to prepare products for high-throughput identification of long-grain wheat varieties.

Description

technical field [0001] The invention relates to the application of KASP molecular marker, in particular to a KASP molecular marker and application related to the length of wheat grains. Background technique [0002] As an important food crop in the world, wheat provides about one-fifth of the calories consumed by human beings. However, with the continuous increase of the population and the gradual reduction of the cultivated land area, the intensification of the contradiction, coupled with the frequent occurrence of extreme climatic phenomena brought about by global warming, has seriously restricted the production of wheat. Therefore, increasing wheat production is an urgent task to ensure global food and nutrition security. [0003] Wheat yield is mainly composed of grain weight, number of grains per panicle and number of panicles per unit area. The grain weight of wheat is mainly determined by the grain size, and the grain size of wheat can be further decomposed into suc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2563/107
Inventor 赵杰张颖君胡梦芸孙丽静赵芸杨锴李辉
Owner 河北省农林科学院粮油作物研究所
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