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Passivator applied to TMV passivation in tobacco seedling raising period and application of passivator

A passivating agent and a technology for tobacco seedlings are applied in the field of tobacco disease prevention and control to achieve the effects of improving activity and improving activity

Pending Publication Date: 2022-06-28
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Passivator applied to TMV passivation in tobacco seedling raising period and application of passivator
  • Passivator applied to TMV passivation in tobacco seedling raising period and application of passivator
  • Passivator applied to TMV passivation in tobacco seedling raising period and application of passivator

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Embodiment 1

[0024] Example 1 Test materials and methods

[0025] experiment material

[0026] Preparation of passivating agent: according to the active ingredients of 10% skimmed milk (skimmed milk, w / v) + 3% trisodium phosphate + 0.1% Tween 20, the passivating agent of the present invention is prepared;

[0027] The tested tobacco varieties are 'Kuban Sanshengyan' and 'K326', which were cultivated in the greenhouse (25-30°C) of the Jimo Experimental Base of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences for 16 hours in the light and 8 hours in the dark to the 7-8 leaf stage. Healthy and consistent growth of tobacco seedlings were selected for testing.

[0028] The source of TMV was provided by the virus research group of the Plant Protection Center, Tobacco Research Institute, Chinese Academy of Agricultural Sciences.

[0029] Preparation method of test virus inoculum: fresh TMV diseased leaves were ground with 0.01mol / L phosphate buffer solution (PBS, p...

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Abstract

The invention discloses a passivator applied to TMV passivation in the tobacco seedling stage and application thereof.The passivator is prepared by taking skim milk and trisodium phosphate as main active ingredients, and the passivation effect of a compound preparation of the skim milk and the trisodium phosphate on a substrate and a leaf cutter polluted by TMV in the tobacco floating seedling raising process is measured through a spot method; a transmission electron microscope is utilized to observe the influence of the passivator on virions, and meanwhile, real-time qPCR is adopted to quantitatively detect the TMV content and the relative expression quantity of host defense proteins. Results show that diluents with different concentrations can significantly passivate the TMV in the matrix and inhibit the infection and morbidity of the TMV on tobacco seedlings, the content of the TMV in the matrix is reduced by 74.09%-90.26%, and the relative control effect reaches 54.83%-85.45%.

Description

technical field [0001] The invention belongs to the field of tobacco disease prevention and control, and specifically provides a passivating agent for TMV passivation at the seedling stage of tobacco and its application, wherein the passivating agent is prepared by using skim milk and trisodium phosphate as main active components. Background technique [0002] Tobacco floating seedling technology has been widely used in domestic tobacco production. The virus disease caused by Tobacco Mosaic Virus (TMV) has become the main disease in the seedling period, and it is easy to spread rapidly through agricultural operations such as cutting leaves, resulting in a large number of hidden diseases and viruses in transplanted seedlings, and large-scale mosaic disease in the field after transplanting. area occurs. Studies have shown that diseased bodies, seedling trays, substrates and leaf-cutting tools are important primary infection sources for TMV transmission. At present, the disin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/26A01N63/10A01P21/00A01H1/00
CPCA01N59/26A01N63/10A01N25/002A01H1/126
Inventor 贾海江王杰韦建玉黄崇峻闫慧峰张纪利李俊霖农世英金亚波欧清华
Owner CHINA TOBACCO GUANGXI IND