Fluorescence detection device and fluorescence detection method

Abstract

The invention relates to a fluorescence detection device and a fluorescence detection method, the fluorescence detection device comprises a fluorescence excitation assembly, and the fluorescence excitation assembly comprises: a first housing; the light-emitting module is mounted in the first accommodating cavity; the device comprises a first excitation dichroscope, a second excitation dichroscope and a third excitation dichroscope, the first excitation dichroscope and the second excitation dichroscope are arranged side by side in a first direction, and the third excitation dichroscope and the second excitation dichroscope are arranged side by side in a second direction perpendicular to the first direction. The first excitation dichroscope, the second excitation dichroscope and the third excitation dichroscope are parallel to one another; and the focusing device is positioned on one side, opposite to the first excitation dichroscope, of the second excitation dichroscope in the first direction. According to the fluorescence detection device, four different fluorescence excitation channels can be formed only by three dichroscopes, so that the structure of the fluorescence excitation assembly is remarkably simplified.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a fluorescence detection device and a fluorescence detection method. Background technique [0002] The polymerase chain reaction (polymerase chain reaction), referred to as PCR, is a method for rapid amplification of DNA or RNA fragments to be determined in vitro. It consists of three basic reaction steps: denaturation-annealing-extension. Polymerase chain reaction analyzer (polymerase chain reaction analyzer) referred to as PCR instrument, based on the principle of PCR technology, simulates the replication process of DNA or RNA, and specifically amplifies known sequences in the presence of templates, primers, polymerases, etc. Instruments and equipment for its detection and analysis. Real-time fluorescent quantitative PCR analysis is developed on the basis of PCR technology. Real-time fluorescent quantitative PCR analysis adds specific fluorescent dyes to the PCR re...

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Problems solved by technology

[0005] Based on this, it is necessary to provide a fluorescence detection device and a fluorescence detection method for the problem that the structure of the multi-flux fluorescence quantitative detection device is relatively complicated. The fluorescence detection device and the fluorescence detection method can realize multi-pass detection while simplifying the structure Quantitative detection of technical effects

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